CAJ | 학술논문

选取具有杀虫效果的苏云金芽孢杆菌(Bt)和抗病效果的枯草芽孢杆菌(SD)进行原生质体融合.通过对融合条件的摸索,得出Bt的最佳培养时间为5 h.最佳酶解浓度为0.8 mg/mL,最佳酶解时间为70 min;SD最佳培养时间为5 h,最佳酶解浓度为0.6 mg/mL,最佳酶解时间为40 min.通过检测,试验得出的融合子具备双亲的功效.
선취구유살충효과적소운금아포간균(Bt)화항병효과적고초아포간균(SD)진행원생질체융합.통과대융합조건적모색,득출Bt적최가배양시간위5 h.최가매해농도위0.8 mg/mL,최가매해시간위70 min;SD최가배양시간위5 h,최가매해농도위0.6 mg/mL,최가매해시간위40 min.통과검측,시험득출적융합자구비쌍친적공효.
The insecticidal Bacillus thuringieusis (Bt) and disease-resistant Bacillus subtilis (SD) were selected for protoplast fusion. Through the exploration for fusion condition, the optimum cultural conditions for Bt were concluded as follows: the optimum cultural time was 5 h, the optimum concentration of enzymolysis was 0.8 mg/ml, and the opfimmtimeof enzymolysis was 70 mins. The optimum cultural conditions for SD were concluded as follows: the optimum cultural time was 5 h, the optimum concentration of enzymolysis was 0.6 mg/ml, and the optimum time of enzymolysis was 40 mins. Through the test, the results indicated that the fusant, which got from the experiment, have the efficacies of both parents.