CAJ | 학술논문

目的观察大肠癌患者和接种Colon26细胞小鼠的Th1/Th2平衡偏移情况,同时以IL-21调整小鼠Th1/Th2平衡,观察调节细胞免疫平衡对大肠癌的影响.方法随机选取30例大肠癌患者,采用流式细胞仪测定Th1/Th2细胞比值及血清中IFN-γ和IL-4浓度.另于小鼠爪垫内侧皮下接种高分泌IL-21的Colon26/IL-21细胞,对比观察小鼠肿瘤生长情况及对机体免疫平衡状态的影响.结果 ①30例大肠癌患者和30例正常人Th1/Th2比值分别为0.74±0.27和1.26±0.9;血清IFN-γ浓度的测定值分别为(6.26±1.43)和(9.18±2.31)pg/ml;IL-4浓度的测定值分别为(8.88±1.30)和(5.20±1.71)pg/ml;IFN-γ/IL-4比值的测定值分别为0.70±0.10和1.82±0.35,两者差异明显.②接种Colon26/IL-21细胞组小鼠肿瘤持续生长至16 d后逐渐缩小,小鼠血清中IFN-γ浓度明显高于对照组;接种Colon26细胞组小鼠肿瘤持续生长增大,血清中IL-4浓度高于Colon26/IL-21细胞组;免疫平衡向细胞免疫方向转变.结论大肠癌患者与正常人相比细胞免疫功能下降,免疫平衡(Th1/Th2平衡)向Th2型偏移;IL-21可以增强Th1型细胞免疫功能,调节Th1/Th2细胞比例,纠正免疫失衡,有效发挥杀灭肿瘤细胞.
목적 관찰대장암환자화접충Colon26세포소서적Th1/Th2평형편이정황,동시이IL-21조정소서Th1/Th2평형,관찰조절세포면역평형대대장암적영향.방법 수궤선취30례대장암환자,채용류식세포의측정Th1/Th2세포비치급혈청중IFN-γ화IL-4농도.령우소서조점내측피하접충고분비IL-21적Colon26/IL-21세포,대비관찰소서종류생장정황급대궤체면역평형상태적영향.결과 ①30례대장암환자화30례정상인Th1/Th2비치분별위0.74±0.27화1.26±0.9;혈청IFN-γ농도적측정치분별위(6.26±1.43)화(9.18±2.31)pg/ml;IL-4농도적측정치분별위(8.88±1.30)화(5.20±1.71)pg/ml;IFN-γ/IL-4비치적측정치분별위0.70±0.10화1.82±0.35,량자차이명현.②접충Colon26/IL-21세포조소서종류지속생장지16 d후축점축소,소서혈청중IFN-γ농도명현고우대조조;접충Colon26세포조소서종류지속생장증대,혈청중IL-4농도고우Colon26/IL-21세포조;면역평형향세포면역방향전변.결론 대장암환자여정상인상비세포면역공능하강,면역평형(Th1/Th2평형)향Th2형편이;IL-21가이증강Th1형세포면역공능,조절Th1/Th2세포비례,규정면역실형,유효발휘살멸종류세포.
Objective To observe the level of Th1/Th2 balance in colorectal carcinoma patient and Colon26 mice transplanted with Colon26 cells in clawpad by flow cytometry and ELISA method;at the same time,the effect of the Thl/Th2 balance of mice by secreting IL-21 cytokine and how the adjustment of immune balance effect the tumor growth of the mice.Methods 30 cases of colorectal carcinoma patients were selected in.The ratio of Th1/Th2 was detected by flow cytometry.The levels of IFN-γ and IL-4 of the serum were measured.Colon26/IL-21cells were transplanted in clawpad of mice.Results ①The level of Th1/Th2 in colorectal carcinoma patients and healthy adults was 0.74±0.27 and 1.26±0.9;The level of IFN-γ in the serum of colorectal carcinoma patients and healthy adults was (6.26±1.43) and (9.18±2.31)pg/ml;The level of IL-4 in the serum of colorectal carcinoma patients and healthy adults was (8.88±1.30) and (5.20±1.71)pg/ml.The difference of the data between two groups was significant(P<0.05).The value of tumor of the mice which was transplanted with Colon26 cells in clawpad decreased gradually and the level of IL-4 in the serum was higher than that of Colon26/IL-21cells.Conclusions Compared with normal immune (balance Th1/Th2 balance) of colorectal carcinoma patients is inclined to Th2 type;IL-21 can enhance the function of Th1 cells and regulate the ratio of Th1/Th2 cells,thus correcting the immune imbalance.Regulating the immune balance can be expected to become a major means of colorectal carcinoma immunotherapy.