CAJ | 학술논문

通过离子交换层析和凝胶过滤层析,从黑眶蟾蜍(Bufo melanostictus)血清中得到其白蛋白,命名为Bufom-SA.该蛋白有抑制胰蛋白酶水解小肽底物活性的作用,但对其他丝氨酸蛋白酶如凝血酶、糜蛋白酶、弹性蛋白酶及枯草杆菌蛋白酶无抑制.Bufom-SA对温度、pH及还原剂二硫苏糖醇的耐受性较强,3种因素对其胰蛋白酶抑制活性影响不大.黑眶蟾蜍血清白蛋白具有稳定的胰蛋白酶抑制活性,可能在黑眶蟾蜍防御天敌捕食的过程中发挥重要作用.
통과리자교환층석화응효과려층석,종흑광섬서(Bufo melanostictus)혈청중득도기백단백,명명위Bufom-SA.해단백유억제이단백매수해소태저물활성적작용,단대기타사안산단백매여응혈매、미단백매、탄성단백매급고초간균단백매무억제.Bufom-SA대온도、pH급환원제이류소당순적내수성교강,3충인소대기이단백매억제활성영향불대.흑광섬서혈청백단백구유은정적이단백매억제활성,가능재흑광섬서방어천활포식적과정중발휘중요작용.
Serum albumin of Bufo melanostictus was obtained through the steps of mixing ion-exchange and gel filtration chromatography,and named Bufom-SA.Bufom-SA inhibited trypsin,but no inhibitory effect on thrombin,chymotrypsin,elastase and subtilisin were observed under the assay conditions.Bufom-SA exhibited the trypsin inhibitory stability against temperature,pH,and DTT,which suggested that skin albumin possessed stable trypsin inhibitory activity,and maybe played an important role in defending predators.