CAJ | 학술논문

目的观察伏格特-小柳-原田(VKH)综合征患者治疗前以及治疗1个月后外周血中CD4+CD25+FOXP3+T细胞的比例变化.方法采集15例VKH综合征患者治疗前以及系统的糖皮质激素治疗1个月后的外周血,分离淋巴细胞.采用细胞表面标志物CD4、CD25以及特征性的细胞核内转录因子叉状头/翅膀状螺旋转录因子(FOXP3)的抗体标记CD4+CD25+调节性T细胞,流式细胞仪检测CD4+CD25+FOXP3+T细胞的比例,并与15例以健康志愿者为正常对照组的外周血中的该亚群细胞水平进行比较.结果外周血中CD4+CD25+FOXP3+T细胞占CD4+细胞的比例,治疗前患者组为(0.30±0.19)%,正常对照组为(1.41±0.52)%,二者比较,差异有统计学意义(t=7.665,P＜0.01);治疗1个月后,患者组为(1.28士0.54)%,接近正常对照组.另有2例患者在治疗1个月后外周血中CD4+CD25+T细胞比例异常升高.结论 VKH综合征患者外周血中CD4+CD25+FOXP3+T细胞的比例治疗前显著低于正常对照组,治疗后与正常对照组接近,表明VKH综合征的发病可能与患者外周血中天然性CD4+CD25+调节性T细胞的比例下降有关.
목적 관찰복격특-소류-원전(VKH)종합정환자치료전이급치료1개월후외주혈중CD4+CD25+FOXP3+T세포적비례변화.방법 채집15례VKH종합정환자치료전이급계통적당피질격소치료1개월후적외주혈,분리림파세포.채용세포표면표지물CD4、CD25이급특정성적세포핵내전록인자차상두/시방상라선전록인자(FOXP3)적항체표기CD4+CD25+조절성T세포,류식세포의검측CD4+CD25+FOXP3+T세포적비례,병여15례이건강지원자위정상대조조적외주혈중적해아군세포수평진행비교.결과 외주혈중CD4+CD25+FOXP3+T세포점CD4+세포적비례,치료전환자조위(0.30±0.19)%,정상대조조위(1.41±0.52)%,이자비교,차이유통계학의의(t=7.665,P＜0.01);치료1개월후,환자조위(1.28사0.54)%,접근정상대조조.령유2례환자재치료1개월후외주혈중CD4+CD25+T세포비례이상승고.결론 VKH종합정환자외주혈중CD4+CD25+FOXP3+T세포적비례치료전현저저우정상대조조,치료후여정상대조조접근,표명VKH종합정적발병가능여환자외주혈중천연성CD4+CD25+조절성T세포적비례하강유관.
Objective To observe the proportion changes of CD4+CD25+FOXP3+ T cells in peripheral blood of patients with Vogt-Koyanagi-Harada disease(VKH)before and after one month of treatment. Methods The peripheral blood samples from 15 patients with VKH disease before and after one month of treatment by glucocorticoid,and from 15 healthy volunteers were collected,and lymphoeytes were separated from them.CD4+CD25+regulatory T cells were Iabeled by antibodies of cell surface marker CD4、CD25 and transcription factor FOXP3.The proportion of CD4+CD25+FOXP3+ T cells were detected by flow cytometry. Results Before the treatment,the percentage of CD4+ CD25+FOXP3+ T cells in periphery blood was(0.30±0.19)%of CD4+ cell in VKH patients,and(1.41±0.52)%in control group,the difference was statistically significant(t=7.665,P＜0.01);after one month of treatment,the VKH patients group was(1.28±0.54)%which close to the control group.However there were two patients whose CD4+ CD25+ T cells inereased extraordinarily after one month of treatment. Conclusions The proportion of CD4+ CD25+ FOCP3+ T cells in periphery blood in VKH patients were lower than control group obviously before treatment,but were close to eontrol group after treatment.Those results indicated that VKH diseases may be associated with the decreased proportion of CD4+ CD25+ regulatory T cells.