中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2004年
22期
4634-4635
,共2页
满勇%李红伟%杨波%孙剑瑞%张清勇%宋来君%赵新利%杜英%阮丽荣%张志强%冯祖荫
滿勇%李紅偉%楊波%孫劍瑞%張清勇%宋來君%趙新利%杜英%阮麗榮%張誌彊%馮祖蔭
만용%리홍위%양파%손검서%장청용%송래군%조신리%두영%원려영%장지강%풍조음
神经节苷脂(GM-1)%干细胞%胚胎
神經節苷脂(GM-1)%榦細胞%胚胎
신경절감지(GM-1)%간세포%배태
背景:近年来研究表明神经节苷脂(gangliosides,GM-1)能够修复中枢神经系统中神经元的损伤.目的:观察不同剂量神经节苷脂(GM-1)对神经干细胞增殖和分化的影响.设计:完全随机对照的开放实验.地点与材料:研究地点为郑州市第二人民医院脑外科和郑州大学医学生物工程研究所.材料为胎龄10周流产胚胎脑组织.方法与主要观察指标:从人胚胎脑组织分离出神经干细胞,培养于含bFGF和B27的DMEM/F12细胞营养液中,实验组加入不同浓度的GM-1,用MTT比色分析法测定细胞增殖能力.用含3%血清的DMEM/F12营养液诱导细胞分化,每间隔6h于倒置相差显微镜下观察细胞分化情况.结果:5 d,10 dMTT比色显示:bFGF(20mg/L)+B27+DMEM/F12和GM-1(33.5mg/L)+B27+DMEM/F12两组间t检验,P值均＞0.05;GM-1(33.5mg/L)+B27+DMEM/F12与B27+DMEM/F12两组间t检验,P值均＜0.05;GM-1(0.335 mg/L)+B27+DMEM/F12,GM-1(3.35 mg/L)+B27+DMEM/F12与B27+DMEM/F12间t检验,P值均＞0.05.分化实验发现,接种6 h后,(3%)血清+DMEM/F12组神经球周边可见明显的细胞突起,呈放射状向周围伸出,而3个实验组和阴性对照组的细胞突起短且细小,随着培养时间的延长,各组的细胞突起均逐渐伸长,3个实验组分化能力低于对照组,与阴性对照组之间无明显差异.结论:GM-1能够维持神经干细胞的原始神经状态,促进神经干细胞的增殖,对神经干细胞无明显的诱导分化作用.
揹景:近年來研究錶明神經節苷脂(gangliosides,GM-1)能夠脩複中樞神經繫統中神經元的損傷.目的:觀察不同劑量神經節苷脂(GM-1)對神經榦細胞增殖和分化的影響.設計:完全隨機對照的開放實驗.地點與材料:研究地點為鄭州市第二人民醫院腦外科和鄭州大學醫學生物工程研究所.材料為胎齡10週流產胚胎腦組織.方法與主要觀察指標:從人胚胎腦組織分離齣神經榦細胞,培養于含bFGF和B27的DMEM/F12細胞營養液中,實驗組加入不同濃度的GM-1,用MTT比色分析法測定細胞增殖能力.用含3%血清的DMEM/F12營養液誘導細胞分化,每間隔6h于倒置相差顯微鏡下觀察細胞分化情況.結果:5 d,10 dMTT比色顯示:bFGF(20mg/L)+B27+DMEM/F12和GM-1(33.5mg/L)+B27+DMEM/F12兩組間t檢驗,P值均＞0.05;GM-1(33.5mg/L)+B27+DMEM/F12與B27+DMEM/F12兩組間t檢驗,P值均＜0.05;GM-1(0.335 mg/L)+B27+DMEM/F12,GM-1(3.35 mg/L)+B27+DMEM/F12與B27+DMEM/F12間t檢驗,P值均＞0.05.分化實驗髮現,接種6 h後,(3%)血清+DMEM/F12組神經毬週邊可見明顯的細胞突起,呈放射狀嚮週圍伸齣,而3箇實驗組和陰性對照組的細胞突起短且細小,隨著培養時間的延長,各組的細胞突起均逐漸伸長,3箇實驗組分化能力低于對照組,與陰性對照組之間無明顯差異.結論:GM-1能夠維持神經榦細胞的原始神經狀態,促進神經榦細胞的增殖,對神經榦細胞無明顯的誘導分化作用.
배경:근년래연구표명신경절감지(gangliosides,GM-1)능구수복중추신경계통중신경원적손상.목적:관찰불동제량신경절감지(GM-1)대신경간세포증식화분화적영향.설계:완전수궤대조적개방실험.지점여재료:연구지점위정주시제이인민의원뇌외과화정주대학의학생물공정연구소.재료위태령10주유산배태뇌조직.방법여주요관찰지표:종인배태뇌조직분리출신경간세포,배양우함bFGF화B27적DMEM/F12세포영양액중,실험조가입불동농도적GM-1,용MTT비색분석법측정세포증식능력.용함3%혈청적DMEM/F12영양액유도세포분화,매간격6h우도치상차현미경하관찰세포분화정황.결과:5 d,10 dMTT비색현시:bFGF(20mg/L)+B27+DMEM/F12화GM-1(33.5mg/L)+B27+DMEM/F12량조간t검험,P치균＞0.05;GM-1(33.5mg/L)+B27+DMEM/F12여B27+DMEM/F12량조간t검험,P치균＜0.05;GM-1(0.335 mg/L)+B27+DMEM/F12,GM-1(3.35 mg/L)+B27+DMEM/F12여B27+DMEM/F12간t검험,P치균＞0.05.분화실험발현,접충6 h후,(3%)혈청+DMEM/F12조신경구주변가견명현적세포돌기,정방사상향주위신출,이3개실험조화음성대조조적세포돌기단차세소,수착배양시간적연장,각조적세포돌기균축점신장,3개실험조분화능력저우대조조,여음성대조조지간무명현차이.결론:GM-1능구유지신경간세포적원시신경상태,촉진신경간세포적증식,대신경간세포무명현적유도분화작용.
BACKGROUND: Recent research shows that gangliosides(GM-1 ) can repair the damaged neuron of central nervous system.OBJECTIVE: To observe the effects on proliferation and differentiation of nerve stem cells by different dose of GM-1.DF SIGN: Completely randomized case-control study.SETTING and PARTICIPANTS: The experiment was performed in the Department of Cerebral Surgery, Second People' s Hospital of Zhengzhou and Bioengineering Research Institute of Zhengzhou University. Brain tissues of embryo with ten weeks gestational age were collected.METHODS and MAIN OUTCOME MEASURES: Nerve stem cells were separated from embryonic brain tissue and cultured in nutritional solution DMEM/F12 which contained bFGF and B27. GM-1 with different concentrations was added into experimental groups respectively to assay the cell proliferation by MTT colorimetric analysis. DMEM/F12 nutrient solution which contained 3% of blood serum was used to induce cell differentiation. Inverted phase contrast microscope was used to observe the differential situation of cells every 6 hours.RESULTS: MTT color matching in 5th and 10th day: t test was done between bFGF(20 ng/L) + B27 + DMEM/F12 and GM-1 (33.5 mg/L) + B27 +DMEM/F12, P ＞ 0. 05; the t test between GM-1 (33.5 mg/L) + B27 +DMEM/F12 and B27 +DMEM/F12, P ＜ 0. 05; t test between GM-1(0.335 ng/L) +B27+DMEM/F12, GM-1(3.35 mg/L) +B27 +DMEM/F12 and B27 + DMEM/F12, P ＞ 0.05. The differential experiment showed that there were obvious protuberances on the margin of nerve cell ball which presented radial shape. However, the protuberance of cells in three experimental groups and negative control group was short and thin. The protuberance gradually increased with the increase of culture time. There differential ability of three experimental groups was weaker than that of control groups. There was no difference between them and negative control group.CONCLUSION: GM-1 can maintain the progenitornerve states of nerve stem cells and stimulate the proliferation. However, there is no obvious inductive effect on nerve stem cells.
