CAJ | 학술논문

为了进一步研究花药花粉发育过程,我们通过EMS诱变,筛选到拟南芥雄性不育突变体zy1511.遗传分析表明,zy1511为隐性单位点突变.细胞学观察表明,突变体花药中小孢子从四分体释放出后绒毡层并没有开始退化,花药发育后期绒毡层依然部分存在,说明突变体花药绒毡层退化比野生型的要迟,因此,小孢子不能发育成正常花粉粒.利用图位克隆的方法将zy1511定位于第一条染色体上分子标记F25P12和T8L23之间134kb的区间内.本项工作为zy1511基因的克隆及对花粉发育功能分析奠定了基础.目前尚未见到该区间内雄性不育基因的报道.因此,zy1511是控制花粉发育的尚未发现的关键基因.
위료진일보연구화약화분발육과정,아문통과EMS유변,사선도의남개웅성불육돌변체zy1511.유전분석표명,zy1511위은성단위점돌변.세포학관찰표명,돌변체화약중소포자종사분체석방출후융전층병몰유개시퇴화,화약발육후기융전층의연부분존재,설명돌변체화약융전층퇴화비야생형적요지,인차,소포자불능발육성정상화분립.이용도위극륭적방법장zy1511정위우제일조염색체상분자표기F25P12화T8L23지간134kb적구간내.본항공작위zy1511기인적극륭급대화분발육공능분석전정료기출.목전상미견도해구간내웅성불육기인적보도.인차,zy1511시공제화분발육적상미발현적관건기인.
With an aim of the genetic dissection of anther and pollen development, we identified an Arabidopsis mutant line named zy1511 by ethyl-methane sulphonate (EMS) mutagenesis. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene zy1511. Cytological studies of anther at various developmental stages indicated that the mutant anther tapetum did not start degenerating after microspores released from the tetrads. Part of the mutant anther tapetum still existed at late stages of anther development,indicating that mutant tapetum degenerated later than that of the wild-type. So the mutant microspores could not develop into normal pollen grains in the anther. For the further genetic analysis and the map-based cloning of gene zy1511, we have mapped it to a region of 134 kb between molecular markers F25P12 and T8L23 on chromosome 1 using a map -based cloning strategy. As no male sterile genes have been reported in this region, we believe that zy1511 is an undiscovered key gene involved in pollen development.