CAJ | 학술논문

背景:为颌骨缺损患者选择适宜的骨移植材料替代自体骨是当前研究的热点.目的:观察纳米羟基磷灰石/胶原/聚乳酸与兔骨髓间充质干细胞复合物用于修复兔下颌骨缺损的能力,比较其与自体骨修复及单纯纳米羟基磷灰石/胶原/聚乳酸修复的差异.设计、时间及地点:随机对照动物实验,于2007-03/10在锦州市中心医院动物实验室完成.材料:40只新西兰兔随机分成纳米羟基磷灰石/胶原/聚乳酸复合骨髓基质干细胞填充组(简称复合组)、自体骨填充组、单纯支架材料填充组及空白对照组,每组10只.方法:在兔下颌骨体部制造大小为15 mm×15 mm的全厚骨缺损模型.复合组于缺损处植入骨髓基质十细胞与纳米羟基磷灰石/胶原/聚乳酸体外联合培养14 d的复合物:自体骨填充组于缺损处植入自体髂骨;单纯支架材料充填组于缺损处植入纳米羟基磷灰石/胶原/聚乳酸:空白对照组不作任何植入.主要观察指标:分别于植入后1,3,6个月进行骨密度检测及组织学染色观察,根据检测结果评价骨修复效果.结果:复合组与自体骨填充组骨密度比较,差异无显著性(P＞0.05),且均高于单纯支架材料充填组及空白对照组(p＜0.01).复合组和自体骨填充组材料植入后6个月时见,新生骨组织渐成熟.呈大块状,桥接缺损断端,支架材料已所剩无几.单纯支架材料充填组植入后6个月时见,植入区骨小梁增多,但仍见较多纤维组织嵌于其中,易见未降解完全的支架材料.结论:纳米羟基磷灰石/胶原/聚乳酸与骨髓间充质干细胞复合物修复下颌骨缺损效果与自体骨修复相似,均优于单纯支架材料修复.
배경:위합골결손환자선택괄의적골이식재료체대자체골시당전연구적열점.목적:관찰납미간기린회석/효원/취유산여토골수간충질간세포복합물용우수복토하합골결손적능력,비교기여자체골수복급단순납미간기린회석/효원/취유산수복적차이.설계、시간급지점:수궤대조동물실험,우2007-03/10재금주시중심의원동물실험실완성.재료:40지신서란토수궤분성납미간기린회석/효원/취유산복합골수기질간세포전충조(간칭복합조)、자체골전충조、단순지가재료전충조급공백대조조,매조10지.방법:재토하합골체부제조대소위15 mm×15 mm적전후골결손모형.복합조우결손처식입골수기질십세포여납미간기린회석/효원/취유산체외연합배양14 d적복합물:자체골전충조우결손처식입자체가골;단순지가재료충전조우결손처식입납미간기린회석/효원/취유산:공백대조조불작임하식입.주요관찰지표:분별우식입후1,3,6개월진행골밀도검측급조직학염색관찰,근거검측결과평개골수복효과.결과:복합조여자체골전충조골밀도비교,차이무현저성(P＞0.05),차균고우단순지가재료충전조급공백대조조(p＜0.01).복합조화자체골전충조재료식입후6개월시견,신생골조직점성숙.정대괴상,교접결손단단,지가재료이소잉무궤.단순지가재료충전조식입후6개월시견,식입구골소량증다,단잉견교다섬유조직감우기중,역견미강해완전적지가재료.결론:납미간기린회석/효원/취유산여골수간충질간세포복합물수복하합골결손효과여자체골수복상사,균우우단순지가재료수복.
BACKGROUND:Selection of proper bone transplantation materials to substitute autogenous bone for treatment of jaw bone defect in patients has become the focus of current researches.OBJECTIVE:To observe the ability of tissue engineered bone constructed with nano-hydroxyapatite/collagen/poly(L-lactic acid)(nHAC/PLA)co-cultured with bone marrow stromal cells(BMSCs)in treatment of rabbit mandibular defect.and to make a comparison with autogenous bone and simple nHAC/PLA.DESIGN.TIME AND SETTING:A randomized controlled animal experiment was performed at the Animal Laboratory of Jinzhou Central Hospital between March and October 2007.MATERIALS:A total of 40 New Zealand rabbits were randomized to 4 groups.with 10 rabbits per group:nHAC/PLA co-cultured with rabbit BMSCs(BMSCs/nHAC/PLA),autogenous bone,nHAC/PLA,and control.METHODS:A 15 mm×15 mm full-thickness bone defect was created in rabbit mandible body.In the BMSCs/nHAC/PLA group.bone defect was repaired by nHAC/PLA co-cultured with BMSCs for 14 days in vitro;the bone defect in the autogenous bone and nHAC/PLA groups was treated by autogenous iliac bone and nHAC/PLA.respectively;while the control group received no any treatment.MAIN OUTCOME MEASURES:At 1,3,and 6 months after surgery,bone mineral density(BMD)measurement and histological examination were performed to evaluate the repairing effects of bone defect.RESULTS:The BMSCs/nHAC/PLA group exhibited the BMD similar to the autogenous bone group(P＞0.05),but it was significantly higher compared to the nHAC/PLA group and the blank control group(P＜0.01).At 6 months after surgery.newly formed bone tissue grew towards large bulk and bridged defected stumps.and the seaffold materials were hardly seen in the BMSCs/nHAC/PLA and autogenous bone groups;simultaneously.the nHAC/PLA group exhibited increased trabecular bone with many fibrous tissues and incompletely degraded seaffold materials.CONCLUSION:BMSCs/nHAC/PLA seaffold exhibits the effects similar to autogenous bone.but superior to nHAC/PLA seaffold in the treatment of mandibular defects.