CAJ | 학술논문

昆明种小鼠(雄性,体重20±2 g)随机分为正常对照组和五倍子给药组.给药组每日灌胃五倍子提取物(O.2 mL/10g体重.相当于8 g五倍子生药/1 kg体重)一次,连续给药30天.对照组灌胃等量生理盐水.之后分别提取两组小鼠的肝组织总mRNA.经反转录分别用Cy3,Cy5荧光标记.制备用于芯片杂交的cDNA探针.两种探针等量混合后与小鼠全基因组寡核苷酸芯片杂交.杂交信号经芯片扫描仪获取并用GenePix Pro 4.0软件分析.结果表明,五倍子给药组中有461条基因差异表达,其中上调基因267条,下调基因194条,功能已知基因373条,功能未知基因88条.通过对这些差异表达基因的生物学功能及生物通路分析,它们主要涉及代谢、DNA结合与转录、蛋白质合成与修饰、细胞骨架及黏附因子、细胞周期与分化、离子通道与受体、信号转导、免疫、细胞凋亡等.上述研究结果对分析五倍子肝损伤机制可能具有十分重要的作用.
곤명충소서(웅성,체중20±2 g)수궤분위정상대조조화오배자급약조.급약조매일관위오배자제취물(O.2 mL/10g체중.상당우8 g오배자생약/1 kg체중)일차,련속급약30천.대조조관위등량생리염수.지후분별제취량조소서적간조직총mRNA.경반전록분별용Cy3,Cy5형광표기.제비용우심편잡교적cDNA탐침.량충탐침등량혼합후여소서전기인조과핵감산심편잡교.잡교신호경심편소묘의획취병용GenePix Pro 4.0연건분석.결과표명,오배자급약조중유461조기인차이표체,기중상조기인267조,하조기인194조,공능이지기인373조,공능미지기인88조.통과대저사차이표체기인적생물학공능급생물통로분석,타문주요섭급대사、DNA결합여전록、단백질합성여수식、세포골가급점부인자、세포주기여분화、리자통도여수체、신호전도、면역、세포조망등.상술연구결과대분석오배자간손상궤제가능구유십분중요적작용.
Kunming mice(male,weight 20±2g)were daily intragastric administration of Chinese nutgall extract(0.2 mL/1Og body weight,equal to 8g Chinese nutgall material/1kg body weight)for 30 days.Liver tissue mRNA were extracted from normal control group and Chinese nutgall treated group respectively,then were reversely transcribed to cDNA with dUTP labeled by different fluorescence(Cy3,Cy5)as hybridization probes.Both of the cDNA probes were mixed equally in 20μL of hybridization solution and hybridized with mice complete genome oligonucleotide microarray.The fluorescent signals were acquired by laser scanner and analyzed by GenePix Pro 4.0 software.The biological function analysis and the pathway analysis of differentially expressed genes were performed according to Gene Ontology database.As a result,there were 461 genes differentially expressed in Chinese nutgail treated group,in which 373 genes were function-known and the others were function-unknown.Among the 461 genes,267 genes were up-regulated and the others were down-regulated.The differentially expressed genes were involved in metabolism,DNA binding and transcription,protein synthesis and modification,cell cytoskeleton and cell adhesion,cell cycle and differentiation,ion channels and transporters,signal transduction,immune response and apoptosis of liver cell. The presented work might be quite important for understanding the pathogenic mechanisms of liver injury induced by Chinese nutgall.