麦类作物学报
麥類作物學報
맥류작물학보
JOURNAL OF TRITICEAE CROPS
2009年
6期
1105-1109
,共5页
武英鹏%原宗英%李颖%石保明
武英鵬%原宗英%李穎%石保明
무영붕%원종영%리영%석보명
山西%小麦%白粉病%抗性基因%毒性频率
山西%小麥%白粉病%抗性基因%毒性頻率
산서%소맥%백분병%항성기인%독성빈솔
Shanxi province%Wheat%Erysiphe graminis f . sp. Tritici%Resistant genes%Virulence frequency
为明确小麦白粉病抗性基因在山西省的有效性,在温室条件下,通过闭囊壳释放子囊孢子侵染麦苗发病,利用28个已知抗病基因品种(系)对分离自山西省不同生态区的166个小麦白粉病菌株进行了毒性基因频率测定.结果表明,毒性基因V4a、V4b、V13、V20、V21、VXBD、V2+6、V4+8、V2+Mli、V4b+Mli、V5+6、V1+2+9的出现频率为3.6%～29.7%,其对应抗性基因Pm4a、Pm4b、Pm13、Pm20、Pm21、PmXBD、Pm2+6、Pm4+8、Pm2+Mli、Pm4b+Mli、Pm5+6、Pm1+2+9为目前山西省小麦白粉病菌的有效抗病基因,可供转育利用;毒性基因V1、V2、V3a、V3d、V3f、V6、V17、V19、V2+Ta的出现频率为38.6%～79.4%,其对应抗性基因Pm1、Pm2、Pm3a、Pm3d、Pm3f、Pm6、Pm17、Pm19、Pm2+Ta的利用价值已下降;毒性基因V3b、V3c、V3e、V5、V7、V8的出现频率为92.5%～98.7%,其对应抗性基因Pm3b、Pm3c、Pm3e、Pm5、Pm7、Pm8单独使用无利用价值.对各抗性基因在山西省小麦三个生态区的有效性差异分析认为,各生态区应针对毒性基因的变化动态结合农艺性状选择选用合适的抗性基因进行转育.
為明確小麥白粉病抗性基因在山西省的有效性,在溫室條件下,通過閉囊殼釋放子囊孢子侵染麥苗髮病,利用28箇已知抗病基因品種(繫)對分離自山西省不同生態區的166箇小麥白粉病菌株進行瞭毒性基因頻率測定.結果錶明,毒性基因V4a、V4b、V13、V20、V21、VXBD、V2+6、V4+8、V2+Mli、V4b+Mli、V5+6、V1+2+9的齣現頻率為3.6%～29.7%,其對應抗性基因Pm4a、Pm4b、Pm13、Pm20、Pm21、PmXBD、Pm2+6、Pm4+8、Pm2+Mli、Pm4b+Mli、Pm5+6、Pm1+2+9為目前山西省小麥白粉病菌的有效抗病基因,可供轉育利用;毒性基因V1、V2、V3a、V3d、V3f、V6、V17、V19、V2+Ta的齣現頻率為38.6%～79.4%,其對應抗性基因Pm1、Pm2、Pm3a、Pm3d、Pm3f、Pm6、Pm17、Pm19、Pm2+Ta的利用價值已下降;毒性基因V3b、V3c、V3e、V5、V7、V8的齣現頻率為92.5%～98.7%,其對應抗性基因Pm3b、Pm3c、Pm3e、Pm5、Pm7、Pm8單獨使用無利用價值.對各抗性基因在山西省小麥三箇生態區的有效性差異分析認為,各生態區應針對毒性基因的變化動態結閤農藝性狀選擇選用閤適的抗性基因進行轉育.
위명학소맥백분병항성기인재산서성적유효성,재온실조건하,통과폐낭각석방자낭포자침염맥묘발병,이용28개이지항병기인품충(계)대분리자산서성불동생태구적166개소맥백분병균주진행료독성기인빈솔측정.결과표명,독성기인V4a、V4b、V13、V20、V21、VXBD、V2+6、V4+8、V2+Mli、V4b+Mli、V5+6、V1+2+9적출현빈솔위3.6%～29.7%,기대응항성기인Pm4a、Pm4b、Pm13、Pm20、Pm21、PmXBD、Pm2+6、Pm4+8、Pm2+Mli、Pm4b+Mli、Pm5+6、Pm1+2+9위목전산서성소맥백분병균적유효항병기인,가공전육이용;독성기인V1、V2、V3a、V3d、V3f、V6、V17、V19、V2+Ta적출현빈솔위38.6%～79.4%,기대응항성기인Pm1、Pm2、Pm3a、Pm3d、Pm3f、Pm6、Pm17、Pm19、Pm2+Ta적이용개치이하강;독성기인V3b、V3c、V3e、V5、V7、V8적출현빈솔위92.5%～98.7%,기대응항성기인Pm3b、Pm3c、Pm3e、Pm5、Pm7、Pm8단독사용무이용개치.대각항성기인재산서성소맥삼개생태구적유효성차이분석인위,각생태구응침대독성기인적변화동태결합농예성상선택선용합괄적항성기인진행전육.
In order to understand the validity of the resistant genes to wheat powdery mildew in Shanxi province, in greenhouse condition, cleistothecia released ascospores were used to infect wheat seedling. The frequencies of virulence genes for 166 isolates of Erysiphe graminis f . sp. tritici from different ecological areas of Shanxi province were tested. The results showed that virulence frequency of V4a、V4b、V13、V20、V21、VXBD、V2+6、V4+8、V2+Mli、V4b+Mli、V5+6、V1+2+9 were 3.6%～29.7%, Pm4a,Pm4b,Pm13,Pm20,Pm21,PmXBD,Pm2+6,Pm4+8,Pm2+Mli,Pm4b+Mli,Pm5+6, Pm1+2+9 were effective resistant genes to Erysiphe graminis f.sp. tritici and may be applicated as resistant parents; Virulence frequency of V1、V2、V3a、V3d、V3f、V6、V17、V19、V2+Ta were 38.6%～79.4%,The validity of Pm1、Pm2、Pm3a、Pm3d、Pm3f、Pm6、Pm17、Pm19、Pm2+Ta had decreased; Virulence frequency of V3b、V3c、V3e、V5、V7、V8 were 92.5%～98.7%, Pm3b、Pm3c、Pm3e、Pm5、Pm7、Pm8 had no applicable value in breeding if they were utilized as the only resistant sources. The difference of validity of wheat resistant genes in three wheat ecological areas of Shanxi province was anlyased. Suggestions were proposed for the application of resistant materials based on their validity and agronomic characters.
