中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2010年
3期
397-400
,共4页
马成%马龙%RAUSCH Wolf-dieter
馬成%馬龍%RAUSCH Wolf-dieter
마성%마룡%RAUSCH Wolf-dieter
大花罗布麻%槲皮素-3-O-槐糖苷%MPTP%PD模型%酪氨酸羟化酶%多巴胺能神经保护
大花囉佈痳%槲皮素-3-O-槐糖苷%MPTP%PD模型%酪氨痠羥化酶%多巴胺能神經保護
대화라포마%곡피소-3-O-괴당감%MPTP%PD모형%락안산간화매%다파알능신경보호
poacynum hendersonni%quercetin-3-O-sophoroside%MPTP%PD model%tyrosine hydroxlase%dopaminergic neuroprotection
目的 观察大花罗布麻叶中槲皮素-3-O-槐糖苷和黄酮富集物对MPTP型帕金森症(Parkinson's disease,PD)小鼠模型的多巴胺能(dopaminergic,DA)神经保护作用.方法 以C57/bl6小鼠为研究对象,采用MPTP腹腔注射30 mg·kg~(-1) 连续5 d制备PD模型,口服灌胃给予槲皮素-3-O-槐糖苷25 mg·kg~(-1) 和大花罗布麻叶黄酮富集物250 mg·kg~(-1) ,造模前7 d开始给药.给药结束后进行自主活动和爬杆实验测试动物行为变化,荧光法测定纹状体中多巴胺含量,石蜡切片观察黑质神经细胞病理变化,分析大花罗布麻对多巴胺能神经的保护作用.结果 槲皮素-3-O-槐糖苷25 mg·kg~(-1) 预先给药1 wk,能提升MPTP型PD小鼠的运动能力,提高纹状体中多巴胺含量,减轻黑质神经的损伤,增加酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性细胞表达数量.结论 槲皮素-3-O-槐糖苷能减轻MPTP对PD小鼠的DA神经损伤.
目的 觀察大花囉佈痳葉中槲皮素-3-O-槐糖苷和黃酮富集物對MPTP型帕金森癥(Parkinson's disease,PD)小鼠模型的多巴胺能(dopaminergic,DA)神經保護作用.方法 以C57/bl6小鼠為研究對象,採用MPTP腹腔註射30 mg·kg~(-1) 連續5 d製備PD模型,口服灌胃給予槲皮素-3-O-槐糖苷25 mg·kg~(-1) 和大花囉佈痳葉黃酮富集物250 mg·kg~(-1) ,造模前7 d開始給藥.給藥結束後進行自主活動和爬桿實驗測試動物行為變化,熒光法測定紋狀體中多巴胺含量,石蠟切片觀察黑質神經細胞病理變化,分析大花囉佈痳對多巴胺能神經的保護作用.結果 槲皮素-3-O-槐糖苷25 mg·kg~(-1) 預先給藥1 wk,能提升MPTP型PD小鼠的運動能力,提高紋狀體中多巴胺含量,減輕黑質神經的損傷,增加酪氨痠羥化酶(tyrosine hydroxylase,TH)暘性細胞錶達數量.結論 槲皮素-3-O-槐糖苷能減輕MPTP對PD小鼠的DA神經損傷.
목적 관찰대화라포마협중곡피소-3-O-괴당감화황동부집물대MPTP형파금삼증(Parkinson's disease,PD)소서모형적다파알능(dopaminergic,DA)신경보호작용.방법 이C57/bl6소서위연구대상,채용MPTP복강주사30 mg·kg~(-1) 련속5 d제비PD모형,구복관위급여곡피소-3-O-괴당감25 mg·kg~(-1) 화대화라포마협황동부집물250 mg·kg~(-1) ,조모전7 d개시급약.급약결속후진행자주활동화파간실험측시동물행위변화,형광법측정문상체중다파알함량,석사절편관찰흑질신경세포병리변화,분석대화라포마대다파알능신경적보호작용.결과 곡피소-3-O-괴당감25 mg·kg~(-1) 예선급약1 wk,능제승MPTP형PD소서적운동능력,제고문상체중다파알함량,감경흑질신경적손상,증가락안산간화매(tyrosine hydroxylase,TH)양성세포표체수량.결론 곡피소-3-O-괴당감능감경MPTP대PD소서적DA신경손상.
Aim To study the dopaminergic neuroprotection of quercetin-3-O-sophoroside and total flavonoids derived from Poacynum hendersonni in MPTP-induced mice model of Parkinson's disease.Methods To inject MPTP intraperitoneal to C57/bl6 mouse at 30 mg·kg~(-1)·d~(-1) 5 days for PD model preparation, qercetin-3-O-sophoroside(25 mg·kg~(-1))and total flavonoids(250 mg·kg~(-1))were treated through intragastric administration 7 days before.Watching the mice behavior change through autonomic activities and pole test after final treatment, dopamine content in striatum was determined with fluorescence photometer.The neurons pathological change in substantia nigra(SN)was observed by pafaffin slice and immunohistochemistry staining to analyze the dopaminergic neuroprotective effects of Poacynum hendersonni.Results Treatment of quercetin-3-O-sophoroside for 7 days in advance, can improve PD mice motivation and dopamine concentration in striatum, tyrosine hydroxylase immune-reactive neurons in SN increased as well.Conclusion Quercetin-3-O-sophoroside can attenuate the impairment of MPTP in mice model of PD.
