CAJ | 학술논문

目的:研究慢性高眼压大鼠视网膜髓鞘相关抑制蛋白Nogo-A表达的变化. 方法:成年雄性Wistar大鼠36只随机分为正常对照组6只和慢性高眼压组30只,应用免疫组织化学方法观察慢性高眼压大鼠视网膜不同时点Nogo-A表达的变化.结果:与正常对照组相比,慢性高眼压21d大鼠视网膜变薄,节细胞数量减少(P<0.05); Nogo-A表达增多,与形态学变化相一致(P<0.05).结论:髓鞘相关抑制蛋白Nogo-A在慢性高眼压大鼠视网膜损伤过程中发挥了重要作用.
목적:연구만성고안압대서시망막수초상관억제단백Nogo-A표체적변화. 방법:성년웅성Wistar대서36지수궤분위정상대조조6지화만성고안압조30지,응용면역조직화학방법관찰만성고안압대서시망막불동시점Nogo-A표체적변화.결과:여정상대조조상비,만성고안압21d대서시망막변박,절세포수량감소(P<0.05); Nogo-A표체증다,여형태학변화상일치(P<0.05).결론:수초상관억제단백Nogo-A재만성고안압대서시망막손상과정중발휘료중요작용.
·AIM: To study the expressive variation of Nogo-A on rat retina in the process of chronic ocular hypertension. · METHODS: Thirty-six healthy adult male Wistars were randomly divided into control group (6 rats) and chronic hypertension group (30 rats). Chronic hypertension was created by cauterizing the superficial scleral veins. Immunohistochemistry technique was used to evaluate the expressive varieties of Nogo-A at different time points during the course of chronic ocular hypertension. · RESULTS: The success of the model was indicated by over 40% of increase in the IOP as compared with normal rats. Compared with control group, as time passed chronic hypertension group gradually had detectable morphology changes in the retina. At the 21st day of chronic ocular hypertension, retinas became thinner and the quantity of retinal ganglion cell (RGC) decreased (P<0.05). Assoicated with the morphological changes, the expression of Nogo-A was strongly increased (P<0.05). CONCLUSION: Myelin associated protein Nogo-A plays a part in the process of chronic ocular hypertension.