CAJ | 학술논문

目的研究Wnt信号通路对体外培养的小鼠NIT-1胰岛细胞增殖和凋亡的影响.方法高糖或细胞因子诱导胰岛细胞损伤,给予Wnt3a蛋白孵育,激活Wnt通路.Tunnel及流式细胞术检测细胞凋亡,BrdU检测细胞增殖.实时定量PCR检测同源异型结构域蛋白转录因子2(Pitx2)、T细胞特异性转录因子7类似物2(TCF7L2)mRNA的表达.结果 Wnt蛋白干预组细胞的凋亡减少(P<0.01),增殖增加,促进增殖的相关基因表达上调.结论激活Wnt通路能促进胰岛细胞的增殖及减少其凋亡.
목적 연구Wnt신호통로대체외배양적소서NIT-1이도세포증식화조망적영향.방법 고당혹세포인자유도이도세포손상,급여Wnt3a단백부육,격활Wnt통로.Tunnel급류식세포술검측세포조망,BrdU검측세포증식.실시정량PCR검측동원이형결구역단백전록인자2(Pitx2)、T세포특이성전록인자7유사물2(TCF7L2)mRNA적표체.결과 Wnt단백간예조세포적조망감소(P<0.01),증식증가,촉진증식적상관기인표체상조.결론 격활Wnt통로능촉진이도세포적증식급감소기조망.
Objective To establish whether Wnt-signaling pathway plays a role in mice β-cell function and/or survival in vitro. Methods Mice NIT-1 beta cells were cultured in media with glucose concentration of 33.3 mmol/L and the cytokines interleukin-1β, interferon-γand tumor necrosis factor-α with or without the addition of purified Wnt3a protein in vitro. Subsequently, β-cell apoptosis by Tunnel and flow cytometry, and β-cell proliferation by BrdU were analyzed. Total RNA was extracted to measure gene expressions by real-time PCR.Results Incubations of NIT-1 cells with high glucose and cytokines resulted in an increase in β-cell apoptosis and decrease in β-cell proliferation (P<0.01). In contrast, treatment with Wnt3a protein protected β-cell from glucose and cytokines-induced apoptosis through up-regulating the expressions of above Pitx2、 TCF7L2. Conclusions Wnt-signaling regulates the proliferation of pancreatic β-cell, and protectes β-cell from glucotoxicity and cytokine toxicity with respect to proliferation and apoptosis.