CAJ | 학술논문

目的建立一种可用于定量细菌内毒素浓度的快速检测方法.方法通过在叉指金电极上固定γ-氨丙基三乙氧基硅烷(3-APTES)和戊二醛为载体的多粘菌素B(PMB),使其与细菌内毒素的生物活性物质脂多糖发生反应；检测装置采用基于声表面波技术的双路差分电路,2路输出振荡信号经过混频器进行差分,根据频移量的变化表征内毒素含量.结果频移变化与内毒素含量在75～130 EU/ml范围内基本成线性关系.结论与传统法相比,检测时间缩短到10 min,测试灵敏度达到13.8 kHz/(EU·ml-1),重复性达到90％以上.
목적 건립일충가용우정량세균내독소농도적쾌속검측방법.방법 통과재차지금전겁상고정γ-안병기삼을양기규완(3-APTES)화무이철위재체적다점균소B(PMB),사기여세균내독소적생물활성물질지다당발생반응；검측장치채용기우성표면파기술적쌍로차분전로,2로수출진탕신호경과혼빈기진행차분,근거빈이량적변화표정내독소함량.결과 빈이변화여내독소함량재75～130 EU/ml범위내기본성선성관계.결론 여전통법상비,검측시간축단도10 min,측시령민도체도13.8 kHz/(EU·ml-1),중복성체도90％이상.
Objective To establish a method of rapid detection of bacteria endotoxin based on interdigital gold electrode and surface acoustic wave (SAW).Methods With the carriers of 3-APTES and glutaral pentanedial,Polymyxin B (PMB) was immobilized on interdigital gold electrode,and then reacted with the bacteria endotoxin' s biological active material (lipopolysacchride,LPS) selectively.Double differential circuit based on SAW was employed to complete the detection.Two output oscillation signals were differentiated through mixer and the endotoxin content was described by frequency shift.Results The experimental results indicated that there was a liner relationship between the frequency shift and endotoxin content within the content range of 75-130 EU/ml.Conclusion Comparing with traditional method,the detection time is shortened to 10 minutes,the sensitivity is 13.8 KHz/(EU·mL-1),and repeatability is above 90％.