CAJ | 학술논문

目的研究生物可降解材料硫酸钙对万古霉素和重组人骨形态发生蛋白-2( rhBMP-2)体外缓释特性. 方法利用高效液相色谱分析和抑菌实验检测缓释材料中万古霉素的浓度及活性,利用ELISA试验和ALP实验检测缓释材料中rhBMP-2的浓度及生物活性. 结果 rhBMP-2/万古霉素/硫酸钙药物缓解系统可释放高于55.8 μg/mL万古霉素长达144h,活性达70％以上；可释放活性rhBMP-2长达30d,对骨髓基质干细胞无抑制增殖作用,具有较高的生物安全性. 结论 rhBMP-2/万古霉素／硫酸钙药物缓解系统能缓慢释放活性万古霉素与rhBMP-2,且不会抑制骨髓基质干细胞的增殖,具有较高的临床应用前景.
목적 연구생물가강해재료류산개대만고매소화중조인골형태발생단백-2( rhBMP-2)체외완석특성. 방법 이용고효액상색보분석화억균실험검측완석재료중만고매소적농도급활성,이용ELISA시험화ALP실험검측완석재료중rhBMP-2적농도급생물활성. 결과 rhBMP-2/만고매소/류산개약물완해계통가석방고우55.8 μg/mL만고매소장체144h,활성체70％이상；가석방활성rhBMP-2장체30d,대골수기질간세포무억제증식작용,구유교고적생물안전성. 결론 rhBMP-2/만고매소／류산개약물완해계통능완만석방활성만고매소여rhBMP-2,차불회억제골수기질간세포적증식,구유교고적림상응용전경.
Objective To study the efficacy and safety of in vitro local drug delivery capsules fabricated with the biodegradable material Osteoset(R) plus vancomycin and recombinant human bone morphogenetic protein-2 (rhBMP-2). Methods In vitro local drug delivery capsules were fabricated with the biodegradable material Osteoset(R) plus vancomycin and rhBMP-2.High-Performance Liquid Chromatography (HPLC) and bacterial inhibition tests were performed to detect the concentration and activity of vancomycin in the capsules.Enzyme-linked immunosorbent assay (ELISA) and alkaline phosphatase (ALP) tests were conducted to detect the concentration and activity of rhBMP-2 in the capsules. Results The capsules could release vancomycin above 55.8 μg/mL for up to 144 hours,with an activity of above 70％.They could release active thBMP-2 for up to 30 days,had no inhibition effects on the proliferation of mesenchymal stem cells (MSCs) and had high biological safety. Conclusion Since the capsules of Osteoset(R) plus vancomyein and rhBMP-2 can release high concentrations of active vancomycin and active rhBMP-2 with no inhibition of the proliferation of MSCs,they have good prospects for clinical use.