中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2008年
6期
545-548
,共4页
李百周%杨文涛%周晓燕%范月珍%陆洪芬%施达仁
李百週%楊文濤%週曉燕%範月珍%陸洪芬%施達仁
리백주%양문도%주효연%범월진%륙홍분%시체인
淋巴瘤,黏膜相关淋巴样组织%眼肿瘤%衔接蛋白质类,信号传导%易位,遗传%免疫组织化学%原位杂交,荧光
淋巴瘤,黏膜相關淋巴樣組織%眼腫瘤%銜接蛋白質類,信號傳導%易位,遺傳%免疫組織化學%原位雜交,熒光
림파류,점막상관림파양조직%안종류%함접단백질류,신호전도%역위,유전%면역조직화학%원위잡교,형광
Lymphoma,mucosa-associated lymphoid tissue%Eye neoplasms%Adaptor proteins,signal transducing%Translocation,genetic%Immunohistochemistry%In situ hybridization,fluorescence
目的 研究眼眶及眼附属器黏膜相关(OA-MALT)淋巴瘤中BCL10蛋白表达及染色体易位情况.方法 收集60例OA-MALT淋巴瘤患者的标本,采用免疫组织化学法检测BCL10蛋白的表达,用荧光素原位杂交(FISH)法分别检测AP12-MALT1融合基因、BCL10、MALT1及IgH基因的异常.采用交叉分组设计,对结果进行Fisher精确检验.结果 OA-MALT淋巴瘤中BCLI0蛋白表达的阳性率为85.0%(51/60),其中胞质阳性率为41.7%(25/60),胞核阳性率为43.3%(26/60).FISH结果显示除2例患者有API2.MALTI融合基因外,其余患者的BCL10基因和IgH基因均未发现异常.统计学分析显示OA-MALT淋巴瘤中BCL10蛋白核表达不依赖于染色体异常(P=0.184).结论 OA.MALT淋巴瘤中有较高的BCL10蛋白核表达率,对OA-MALT淋巴瘤的诊断有一定的辅助价值.在全身其他部位MALT淋巴瘤中报道的染色体易位,在OA-MALT淋巴瘤中并不常见.
目的 研究眼眶及眼附屬器黏膜相關(OA-MALT)淋巴瘤中BCL10蛋白錶達及染色體易位情況.方法 收集60例OA-MALT淋巴瘤患者的標本,採用免疫組織化學法檢測BCL10蛋白的錶達,用熒光素原位雜交(FISH)法分彆檢測AP12-MALT1融閤基因、BCL10、MALT1及IgH基因的異常.採用交扠分組設計,對結果進行Fisher精確檢驗.結果 OA-MALT淋巴瘤中BCLI0蛋白錶達的暘性率為85.0%(51/60),其中胞質暘性率為41.7%(25/60),胞覈暘性率為43.3%(26/60).FISH結果顯示除2例患者有API2.MALTI融閤基因外,其餘患者的BCL10基因和IgH基因均未髮現異常.統計學分析顯示OA-MALT淋巴瘤中BCL10蛋白覈錶達不依賴于染色體異常(P=0.184).結論 OA.MALT淋巴瘤中有較高的BCL10蛋白覈錶達率,對OA-MALT淋巴瘤的診斷有一定的輔助價值.在全身其他部位MALT淋巴瘤中報道的染色體易位,在OA-MALT淋巴瘤中併不常見.
목적 연구안광급안부속기점막상관(OA-MALT)림파류중BCL10단백표체급염색체역위정황.방법 수집60례OA-MALT림파류환자적표본,채용면역조직화학법검측BCL10단백적표체,용형광소원위잡교(FISH)법분별검측AP12-MALT1융합기인、BCL10、MALT1급IgH기인적이상.채용교차분조설계,대결과진행Fisher정학검험.결과 OA-MALT림파류중BCLI0단백표체적양성솔위85.0%(51/60),기중포질양성솔위41.7%(25/60),포핵양성솔위43.3%(26/60).FISH결과현시제2례환자유API2.MALTI융합기인외,기여환자적BCL10기인화IgH기인균미발현이상.통계학분석현시OA-MALT림파류중BCL10단백핵표체불의뢰우염색체이상(P=0.184).결론 OA.MALT림파류중유교고적BCL10단백핵표체솔,대OA-MALT림파류적진단유일정적보조개치.재전신기타부위MALT림파류중보도적염색체역위,재OA-MALT림파류중병불상견.
Objective To detect the BCL10 expression and several types of chromosomal translocations [including t (11 ;18)/API2-MALT1 ; t (1 ;14)/IgH- BCL10 and t (14;18)/MALTI-IgH] in ocular adnexal mucosa-associated lymphoid tissue lymphomas (OA-MALT lymphomas). Methods Sixty OA-MALT lymphomas were collected from Cancer Hospital of Fudan University. BCLIO was detected by immunohistochemical studies, and API2-MALT1 fusion gene, BCLIO, MALT1 and IgH chromosomal abnormalities were detected by fluorescent in situ hybridization ( FISH). Fisher's exact test was used to analyze the relation between nuclear BCL10 expression and chromosomal translocation. Results BCL10 expressed in 85.0% (51/60) OA-MALT lymphomas. Among these positive cases, 25 cases (41.7%) were expressed in the cytoplasm, and 26 cases (43.3%) were expressed in the nucleus. FISH results showed that no chromosomal abnormalities related with BCL10 and IgH genes, except 2 cases with API2-MALT1 fusion gene. Under statistic of Fisher exact test, nuclear BCL10 expression and API2-MALT1 fusion gene were two independent factors ( P > 0.05 ). Conclusions BCL10 nuclear expression is common in OAMALT lymphomas and may be used as a potential marker for the diagnosis of MALT lymphomas arising from ocular adnexa. Aberrant chromosomal translocations reported in the other sites MALT lymphomas are rare in OA-MALT lymphomas.
