CAJ | 학술논문

目的探讨糖尿病性勃起功能障碍(ED)大鼠阴茎海绵体平滑肌细胞(CCSM)的体外培养方法,建立糖尿病性ED大鼠CCSM体外培养模型,为糖尿病性ED的研究奠定基础.方法建立糖尿病性ED大鼠动物模型,用改良组织块贴壁法体外培养CCSM并行免疫组织化学染色鉴定,观察细胞形态和生长情况.结果糖尿病性ED大鼠造模成功;用改良组织块法培养糖尿病ED大鼠CCSM,3 d后可见细胞游出.16～18 d细胞长成单层,传代后细胞增殖旺盛,呈"峰-谷"样生长.培养的细胞经α-SM-actin和结蛋白免疫组化染色鉴定为平滑肌细胞,糖尿病性ED大鼠CCSM体外培养模型建立成功.结论改良组织块贴壁法建立糖尿病ED大鼠CCSM体外培养模型简便、稳定;糖尿病ED大鼠CCSM较正常CCSM增殖速度快,在光镜下形态与正常CCSM差别不明显.
목적 탐토당뇨병성발기공능장애(ED)대서음경해면체평활기세포(CCSM)적체외배양방법,건립당뇨병성ED대서CCSM체외배양모형,위당뇨병성ED적연구전정기출.방법 건립당뇨병성ED대서동물모형,용개량조직괴첩벽법체외배양CCSM병행면역조직화학염색감정,관찰세포형태화생장정황.결과 당뇨병성ED대서조모성공;용개량조직괴법배양당뇨병ED대서CCSM,3 d후가견세포유출.16～18 d세포장성단층,전대후세포증식왕성,정"봉-곡"양생장.배양적세포경α-SM-actin화결단백면역조화염색감정위평활기세포,당뇨병성ED대서CCSM체외배양모형건립성공.결론개량조직괴첩벽법건립당뇨병ED대서CCSM체외배양모형간편、은정;당뇨병ED대서CCSM교정상CCSM증식속도쾌,재광경하형태여정상CCSM차별불명현.
Objective To investigate the method for culturing corpus cavemosum smooth muscle cells (CCSMs) derived from diabetic rats with erectile dysfunction (ED) for the study of ED caused by diabetes. Methods CCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture. The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed. Results The primary culture of CCSM was performed successfully, and the cells were seen to migrate from the small tissue pieces 3 days later,reaching nearly confluence in 16-18 days. A typical "hill-valley" growth pattern was noted in the cell passaging.Immunohistochemical staining for α-smooth muscle actin (α-SM-actin) and desmin yielded positive results in the cells.Conclusion The modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED, and the cultured CCSMs display a faster proliferation than normal CCSMs. No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.