CAJ | 학술논문

目的观察和评价针刺及青藤碱(sinomenine)治疗对海洛因成瘾大鼠戒断症状及其杏仁核(amygdala)自发放电及突触素(synaptophysin)表达的影响.方法 75只SD大鼠随机均分5组:对照组、成瘾组、针刺组、青藤碱组和联合治疗组.皮下注射海洛因建立成瘾模型大鼠.3个治疗组分别给予针刺穴位(内关、百会、足三里)、青藤碱肌注及两者联合治疗.评估各组大鼠诱导戒断症状,用电生理技术观察5组大鼠杏仁核自发放电,免疫组织化学技术检测杏仁核突触素表达.结果给予纳洛酮后,大鼠出现典型诱导戒断症状(P<0.01),造模成功;给予针刺及青藤碱治疗后,其戒断症状明显减轻(P<0.01).杏仁核自发放电结果显示:相对于对照组,海洛因成瘾大鼠自发放电低频放电和单个不匀放电比例增多,束簇状和混合型放电比例明显减少(P<0.05),而给予青藤碱或联合治疗的成瘾大鼠放电形式得以逆转(P<0.05),接近对照组水平.免疫组化结果显示,相对于对照组,成瘾组大鼠杏仁核突触素表达增多(P<0.01),给予针刺、青藤碱及联合治疗的大鼠杏仁核突触素的表达明显减少(P<0.01).结论针刺及青藤碱治疗可以减轻海洛因成瘾大鼠的戒断症状,一定程度地纠正成瘾大鼠杏仁核自发放电频率和形式的异常,降低海洛因成瘾大鼠杏仁核突触素的表达,且二者联合应用效果更好.
목적 관찰화평개침자급청등감(sinomenine)치료대해락인성은대서계단증상급기행인핵(amygdala)자발방전급돌촉소(synaptophysin)표체적영향.방법 75지SD대서수궤균분5조:대조조、성은조、침자조、청등감조화연합치료조.피하주사해락인건립성은모형대서.3개치료조분별급여침자혈위(내관、백회、족삼리)、청등감기주급량자연합치료.평고각조대서유도계단증상,용전생리기술관찰5조대서행인핵자발방전,면역조직화학기술검측행인핵돌촉소표체.결과 급여납락동후,대서출현전형유도계단증상(P<0.01),조모성공;급여침자급청등감치료후,기계단증상명현감경(P<0.01).행인핵자발방전결과현시:상대우대조조,해락인성은대서자발방전저빈방전화단개불균방전비례증다,속족상화혼합형방전비례명현감소(P<0.05),이급여청등감혹연합치료적성은대서방전형식득이역전(P<0.05),접근대조조수평.면역조화결과현시,상대우대조조,성은조대서행인핵돌촉소표체증다(P<0.01),급여침자、청등감급연합치료적대서행인핵돌촉소적표체명현감소(P<0.01).결론 침자급청등감치료가이감경해락인성은대서적계단증상,일정정도지규정성은대서행인핵자발방전빈솔화형식적이상,강저해락인성은대서행인핵돌촉소적표체,차이자연합응용효과경호.
Aim To explore the effects of acupuncture and sinomenine on the withdrawal symptoms,on the spontaneous discharge and synaptophysin expression of amygdala of heroin-addicted rats.Methods 75 SD rats were randomly and equally divided into 5 groups consisted of control,heroin-addicted,acupuncture,sinomenine and combined treatment group.The model of heroin-addicted rats were established with heroin subcutaneous injection,which in acupuncture,sinomenine and combined group were subsequently punctured at 3 acupoint(Neiguan,Baihui and Zusanli),intramuscular injected with sinomenine and treated with combined methods(acupuncture and sinomenine)respectively.The withdrawal symptoms of rats in different group were evaluated,and then the spontaneous discharges and synaptophysin expression of amygdala were detected with electrophysiological and immunohistochemical technology respectively.Results The heroin-addicted rats were successfully established,being suggested by typical withdrawal symptoms induced by naloxone(P<0.01 vs control),which were alleviated in the 3 treatment groups(acupuncture,sinomenine and combined ) with varying degree(P<0.01 vs heroin group).The electrophysiological results suggest that the forms of low-frequency and single irregular discharges in amygdala were increase,bunchy/cluster and complex discharges were decreased(P<0.05 vs control),which were reversed apparently in sinomenine and combined treatment groups(P<0.05 vs heroin group),closed to control.The immunohistochemical results show that expression of synaptophysin in amygdala was increased obviously(P<0.01 vs control),which was decreased in the 3 treatment groups(acupuncture,sinomenine and combined),P<0.01 vs addicted group.Conclusion Treatments of acupuncture and sinomenine administration can alleviate withdrawal symptoms of heroin-addicted,and rectify the abnormal spontaneous discharges and expression of synaptophysin in amygdala of heroin-addicted rats for certain degree,especially with combined treatments.