国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2010年
4期
236-238
,共3页
黄美先%朱婧%潘韵峰%冷建杭
黃美先%硃婧%潘韻峰%冷建杭
황미선%주청%반운봉%랭건항
流感病毒A型,H1N1亚型%淋巴细胞亚群%细胞免疫
流感病毒A型,H1N1亞型%淋巴細胞亞群%細胞免疫
류감병독A형,H1N1아형%림파세포아군%세포면역
Influenza A virus,H1N1 subtype%Lymphocyte subsets%Immunity
目的 通过动态观察重型甲型H1N1流感(重症及危重症)患者外周血淋巴细胞的数量变化,探讨患者的免疫病理机制及淋巴细胞亚群检测在甲型H1N1流感诊断中的临床意义.方法 采用流式细胞技术检测2009年10-12月在我院住院治疗的41例重症、危重症甲型H1N1流感患者和同期41例轻症患者的外周血T、B和NK细胞数量.结果 在发病初期,重症及危重症患者外周血总T细胞、CD8+T细胞、NK细胞较轻症甲型H1N1流感患者显著降低(P＜0.01),CD4+T细胞较轻症患者降低(P＜0.05);危重症患者总T细胞、CD4+T细胞、CD8+T细胞、NK细胞低于重症患者(P＜0.01).恢复期重症及危重症甲型H1N1流感患者总T细胞、CD8+T细胞低于轻症患者(P＜0.05),NK细胞低于轻症患者(P＜0.01),B细胞高于轻症患者(P＜0.05).结论 重症及危重症甲型H1N1流感患者存在明显的细胞免疫损伤.
目的 通過動態觀察重型甲型H1N1流感(重癥及危重癥)患者外週血淋巴細胞的數量變化,探討患者的免疫病理機製及淋巴細胞亞群檢測在甲型H1N1流感診斷中的臨床意義.方法 採用流式細胞技術檢測2009年10-12月在我院住院治療的41例重癥、危重癥甲型H1N1流感患者和同期41例輕癥患者的外週血T、B和NK細胞數量.結果 在髮病初期,重癥及危重癥患者外週血總T細胞、CD8+T細胞、NK細胞較輕癥甲型H1N1流感患者顯著降低(P＜0.01),CD4+T細胞較輕癥患者降低(P＜0.05);危重癥患者總T細胞、CD4+T細胞、CD8+T細胞、NK細胞低于重癥患者(P＜0.01).恢複期重癥及危重癥甲型H1N1流感患者總T細胞、CD8+T細胞低于輕癥患者(P＜0.05),NK細胞低于輕癥患者(P＜0.01),B細胞高于輕癥患者(P＜0.05).結論 重癥及危重癥甲型H1N1流感患者存在明顯的細胞免疫損傷.
목적 통과동태관찰중형갑형H1N1류감(중증급위중증)환자외주혈림파세포적수량변화,탐토환자적면역병리궤제급림파세포아군검측재갑형H1N1류감진단중적림상의의.방법 채용류식세포기술검측2009년10-12월재아원주원치료적41례중증、위중증갑형H1N1류감환자화동기41례경증환자적외주혈T、B화NK세포수량.결과 재발병초기,중증급위중증환자외주혈총T세포、CD8+T세포、NK세포교경증갑형H1N1류감환자현저강저(P＜0.01),CD4+T세포교경증환자강저(P＜0.05);위중증환자총T세포、CD4+T세포、CD8+T세포、NK세포저우중증환자(P＜0.01).회복기중증급위중증갑형H1N1류감환자총T세포、CD8+T세포저우경증환자(P＜0.05),NK세포저우경증환자(P＜0.01),B세포고우경증환자(P＜0.05).결론 중증급위중증갑형H1N1류감환자존재명현적세포면역손상.
Objective To observe the changes of blood lymphocyte in severe influenza A (H1N1) patients, and to investigate the immunopathogenesis of the influenza A( H1N1 ) patients and the clinical value of lymphocyte detection in influenza A (H1N1) diagnosis. Methods The changes of blood lymphocyte subs,ets (T cells, B cells, NK cells) were determined by the flow cytometer analysis in41 patients with serious and critical influenza A (H1N1) and 41 patients with mild influenza A(H1N1). Results The counts of total T cells, CD4+ T cells,CD8+ T cells, NK cells all significantly decreased in acute phase of serious and critical patients compared with those in mild patients (total T cells, CD8+T cells,NK cells, P＜0.01;CD4+T cells, P＜0.05). The counts of above cells in critical influenza A ( H1N1 ) patients were less than those in serious patients(P＜0.01). As the conditions improved in serious and critical patients, the counts of total T cells, CD8+T cells and NK cells were still less than those in mild patients(total T cells, CD8+T cells, P＜0.05; NK cells, P ＜ 0.01 ). The counts of B cells were higher than that in mild individuals ( P＜0.05). Conclus The patients with serious and critical influenza A (H1N1) are markedly impaired with cellular immunity.
