国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
8期
692-696
,共5页
洪晓华%杨斌%于魏林%董晓霞%李宏坤%刘建勋
洪曉華%楊斌%于魏林%董曉霞%李宏坤%劉建勛
홍효화%양빈%우위림%동효하%리굉곤%류건훈
益肾颗粒%糖尿病肾病大鼠%Ⅳ型胶原%层黏连蛋白%纤维黏连蛋白
益腎顆粒%糖尿病腎病大鼠%Ⅳ型膠原%層黏連蛋白%纖維黏連蛋白
익신과립%당뇨병신병대서%Ⅳ형효원%층점련단백%섬유점련단백
Yishen granule%Diabetic nephropathy%Type Ⅳ collagen%LN%FN
目的 观察益肾颗粒对糖尿病肾病大鼠肾组织Ⅳ型胶原、层黏连蛋白、纤维黏连蛋白表达的影响,探讨其肾脏保护作用机制.方法 建立单侧肾切除链脲佐菌素Ⅰ型糖尿病肾病大鼠模型,试验分为正常组、单切肾组、DN模型组、益肾颗粒低剂量组(8.1 g/kg)、益肾颗粒高剂量组(16.2 g/kg)、洛汀新组(0.0015 g/kg);正常组、单切肾组、DN模型组给予等体积蒸馏水,均灌胃给药,1次/d,连续16周.观察大鼠肾组织Ⅳ型胶原、层黏连蛋白、纤维黏连蛋白表达及肾小球形态学改变.结果 正常组、单切肾组、DN模型组、益肾颗粒低剂量组、益肾颗粒高剂量组、洛汀新组的Ⅳ型胶原分别(1521.22±415.26)、(1579.22±343.26)、(3402.00±863.39)、(2984.30±674.53)、(2959.15±561.22)、(2918.04±363.96)；层黏连蛋白表达分别为(1968.04±522.17)、(2004.52±417.19)、(3299.04±665.78)、(3116.89±540.10)、(2932.63±528.38)、(2815.89±798.58)；纤维黏连蛋白表达分别为(2614.67±533.82)、(2742.63±562.80)、(3311.41±529.29)、(2993.44±548.66)、(2953.30±535.74)、(2897.41±505.84).益肾颗粒高、低剂量组均可下调肾组织、层黏连蛋白及纤维黏连蛋白的表达,降低三者累积光密度值(均P＜0.05),减轻肾小球系膜细胞、系膜基质增生及系膜区纤维化程度,缩小球囊腔扩张,使毛细血管变清晰,减轻内皮下基底膜增生,有改善肾小球的病理变化作用,并以高剂量组更显著.结论 益肾颗粒对糖尿病肾病模型大鼠的治疗作用可能与抑制肾组织Ⅳ型胶原、层黏连蛋白及纤维黏连蛋白的表达有关.
目的 觀察益腎顆粒對糖尿病腎病大鼠腎組織Ⅳ型膠原、層黏連蛋白、纖維黏連蛋白錶達的影響,探討其腎髒保護作用機製.方法 建立單側腎切除鏈脲佐菌素Ⅰ型糖尿病腎病大鼠模型,試驗分為正常組、單切腎組、DN模型組、益腎顆粒低劑量組(8.1 g/kg)、益腎顆粒高劑量組(16.2 g/kg)、洛汀新組(0.0015 g/kg);正常組、單切腎組、DN模型組給予等體積蒸餾水,均灌胃給藥,1次/d,連續16週.觀察大鼠腎組織Ⅳ型膠原、層黏連蛋白、纖維黏連蛋白錶達及腎小毬形態學改變.結果 正常組、單切腎組、DN模型組、益腎顆粒低劑量組、益腎顆粒高劑量組、洛汀新組的Ⅳ型膠原分彆(1521.22±415.26)、(1579.22±343.26)、(3402.00±863.39)、(2984.30±674.53)、(2959.15±561.22)、(2918.04±363.96)；層黏連蛋白錶達分彆為(1968.04±522.17)、(2004.52±417.19)、(3299.04±665.78)、(3116.89±540.10)、(2932.63±528.38)、(2815.89±798.58)；纖維黏連蛋白錶達分彆為(2614.67±533.82)、(2742.63±562.80)、(3311.41±529.29)、(2993.44±548.66)、(2953.30±535.74)、(2897.41±505.84).益腎顆粒高、低劑量組均可下調腎組織、層黏連蛋白及纖維黏連蛋白的錶達,降低三者纍積光密度值(均P＜0.05),減輕腎小毬繫膜細胞、繫膜基質增生及繫膜區纖維化程度,縮小毬囊腔擴張,使毛細血管變清晰,減輕內皮下基底膜增生,有改善腎小毬的病理變化作用,併以高劑量組更顯著.結論 益腎顆粒對糖尿病腎病模型大鼠的治療作用可能與抑製腎組織Ⅳ型膠原、層黏連蛋白及纖維黏連蛋白的錶達有關.
목적 관찰익신과립대당뇨병신병대서신조직Ⅳ형효원、층점련단백、섬유점련단백표체적영향,탐토기신장보호작용궤제.방법 건립단측신절제련뇨좌균소Ⅰ형당뇨병신병대서모형,시험분위정상조、단절신조、DN모형조、익신과립저제량조(8.1 g/kg)、익신과립고제량조(16.2 g/kg)、락정신조(0.0015 g/kg);정상조、단절신조、DN모형조급여등체적증류수,균관위급약,1차/d,련속16주.관찰대서신조직Ⅳ형효원、층점련단백、섬유점련단백표체급신소구형태학개변.결과 정상조、단절신조、DN모형조、익신과립저제량조、익신과립고제량조、락정신조적Ⅳ형효원분별(1521.22±415.26)、(1579.22±343.26)、(3402.00±863.39)、(2984.30±674.53)、(2959.15±561.22)、(2918.04±363.96)；층점련단백표체분별위(1968.04±522.17)、(2004.52±417.19)、(3299.04±665.78)、(3116.89±540.10)、(2932.63±528.38)、(2815.89±798.58)；섬유점련단백표체분별위(2614.67±533.82)、(2742.63±562.80)、(3311.41±529.29)、(2993.44±548.66)、(2953.30±535.74)、(2897.41±505.84).익신과립고、저제량조균가하조신조직、층점련단백급섬유점련단백적표체,강저삼자루적광밀도치(균P＜0.05),감경신소구계막세포、계막기질증생급계막구섬유화정도,축소구낭강확장,사모세혈관변청석,감경내피하기저막증생,유개선신소구적병리변화작용,병이고제량조경현저.결론 익신과립대당뇨병신병모형대서적치료작용가능여억제신조직Ⅳ형효원、층점련단백급섬유점련단백적표체유관.
Objective To investigate the influence of Yishen granule on the expression of type Ⅳ collagen,laminin (LN)and fibroneetin (FN)in diabetic nephropathy (DN) rats.Methods DN model rats were established by unilateral nephrectomy and intraperltoneal injection of STZ.The experimental rats were divided into six groups:normal group,DN model group,Yishen granule groups in low and high dose (8.1 g/kg、16.2 g/kg) and positive control medicine group with lotensin (0.0015 g/kg).After 16 weeks of treatment,kidney tissues were sampled and pathologically observed through Masson trichrome staining.The proteins of type Ⅳ collagen、LN and FN were determined through immunohistochemical methods.Results The type Ⅳ collagen in normal group,DN model group,Yishen granule groups in low and high dose,and positive control medicine group was (1521.22 ± 415.26),(1579.22 ± 343.26),(3402.00 ± 863.39),(2984.30 ± 674.53),(2959.15 ± 561.22),(2918.04±363.96); LN was (1968.04±522.17),(2004.52±417.19),(3299.04±665.78),(3116.89±540.10),(2932.63 ± 528.38),(2815.89 ± 798.58) ; FN was (2614.67 ± 533.82),(2742.63 ± 562.80),(3311.41 ± 529.29),(2993.44±548.66),(2953.30±535.74),(2897.41 ±505.84) respectively.The level of type Ⅳ collagen、LN and FN expression in Yishen granule group obviously decreased as compared with that in DN model group (P＜0.05).The mesangial cells,basement membrane thickening and endocapillary proliferation in glomerular filtration membrane were significantly alleviated in Yishen granule group.The pathology of kidney was obviously modulated by Yishen granule.Conclusion Yishen granule can significantly inhibits the expression of type Ⅳ collagen、LN and FN in DN rats,which may be the mechanisms for Yishen granule in protecting the DN rat's kidney.
