CAJ | 학술논문

目的观察在应用阿托伐他汀基础上进行间充质干细胞(MSCs)移植对梗死心肌修复的影响及其治疗急性心肌梗死(AMI)的可行性.方法体外获取、培养大鼠骨髓MSCs;结扎左冠状动脉前降支,建立AMI模型.存活大鼠随机分为4组:应用阿托伐他汀并行MSCs移植组(A组)、阿托伐他汀组(B组)、MSCs移植组(C组)、AMI对照组(D组).A、B组术后给予阿托伐他汀10 mg/(kg·d)溶水2 ml,灌喂10 d;C、D组正常喂养.10 d后再次开胸,A、c组分别在梗死部位5个点移植MSCs,每点2×106/50μl;B、D组于相同部位注射同剂量磷酸盐缓冲液.3周后,检测左心室射血分数(LVEF)以及左心室收缩压(LVSP)、左心室舒张末期压(LVEDP)、压力变化最大速率(±dp/dtmax)等指标;免疫组化检测细胞和组织学变化.结果 A、C组与B、D组相比,左心室前壁运动增强,LVEF、LVSP、±dp/dtmax均升高(均P＜0.05),LVEDP降低(P＜0.05),A组较C组改变更明显(P＜0.05).A、C组的梗死心肌内可见5-溴脱氧尿嘧啶(BrdU)标记阳性细胞.对心肌肌钙蛋白T(cTnT)、连接蛋白43(Cx-43)呈现阳性表达.结论应用阿托伐他汀基础上进行MSCs移植对AMI大鼠心功能有明显的改善作用.
목적 관찰재응용아탁벌타정기출상진행간충질간세포(MSCs)이식대경사심기수복적영향급기치료급성심기경사(AMI)적가행성.방법 체외획취、배양대서골수MSCs;결찰좌관상동맥전강지,건립AMI모형.존활대서수궤분위4조:응용아탁벌타정병행MSCs이식조(A조)、아탁벌타정조(B조)、MSCs이식조(C조)、AMI대조조(D조).A、B조술후급여아탁벌타정10 mg/(kg·d)용수2 ml,관위10 d;C、D조정상위양.10 d후재차개흉,A、c조분별재경사부위5개점이식MSCs,매점2×106/50μl;B、D조우상동부위주사동제량린산염완충액.3주후,검측좌심실사혈분수(LVEF)이급좌심실수축압(LVSP)、좌심실서장말기압(LVEDP)、압력변화최대속솔(±dp/dtmax)등지표;면역조화검측세포화조직학변화.결과 A、C조여B、D조상비,좌심실전벽운동증강,LVEF、LVSP、±dp/dtmax균승고(균P＜0.05),LVEDP강저(P＜0.05),A조교C조개변경명현(P＜0.05).A、C조적경사심기내가견5-추탈양뇨밀정(BrdU)표기양성세포.대심기기개단백T(cTnT)、련접단백43(Cx-43)정현양성표체.결론 응용아탁벌타정기출상진행MSCs이식대AMI대서심공능유명현적개선작용.
Objective To study the effects of mesenchymal stem cells(MSCs)transplantation besides of statins on repair of the infarcted myocardium and the possibility of its therapy for acute myocardial infarct(AMI).Methods MSCs of rats were removed and cultured in vitro.The acute myocardial infarction(AMI)model was established through the ligation of left anterior descending coronary artery.The survival rats were randomly divided into 4 groups:the combination of MSCs transplantation and atrovastatin ( group A),atrovastatin group(group B),MSCs transplantation group(group C),and control group(group D).Bats in group A and B were administrated orally with 10mg/Kg.d atrovastatin solution during 10 days after operation;rats in group C and D were fed normally.10 days later,MSCs were injected into 5 points in myocardial infarct region at the concentration of 2×106 cells/50μl/point respectively in group A and C;PBS was injected at the same place and dose in group B and D.3 weeks later,the left ventricular ejection fraction(LVEF),left ventrleular systole pressure(LVSP),left ventricular end diastole pressure (LVEDP),and the maximal rate of left ventricular pressure(+dp/dtmax)were examined respectively.The morphological changes of myocardium at cellular and histological level were measured immunohistochemially.Results Compared with group B and D,the mobility of anterior wall of left ventricle got stronger in group A and C,and the LVEF,LVSP,±dp/dtmax all increased(P＜0.05),while the LVEDP decreased(P＜0.05).Those changes were more significant in group A than in group C(P＜0.05).The 5-bromodeoxyuridine(BrdU)positive cells could be found in infarcted myocardium,and showed positive expression oftroponin(TnT),connexin43(Cx-43).Conclusion Treatment with the combination of MSCs and statins for acute myocardial infarction could obviously promote cardiac function.