中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2008年
9期
866-868
,共3页
冠心病%缺氧诱导因子-1α%侧支循环
冠心病%缺氧誘導因子-1α%側支循環
관심병%결양유도인자-1α%측지순배
Coronary heart disease%Hypoxia-inducible factor-1α%Collateral circulation
目的 探讨冠心病患者体内单个核细胞缺氧诱导因子-1α(HIF-1α)表达水平与侧支循环建立程度的相关性. 方法 选择冠状动脉造影至少有一支血管狭窄超过70%的冠心病患者96例为冠心病组.其中42例有可见的侧支循环,54例未见侧支循环.另选冠状动脉造影正常患者50例作为对照组.免疫组化和Western blot法检测其外周血单个核细胞HIF-1α蛋白含量. 结果 免疫组化和Westem blot两种方法检测冠心病组HIF-1α表达水平均明显高于对照组(均P<0.01),冠心痛组中有侧支循环患者比无侧支循环患者HIF-1α表达更多(均P<0.01),冠状动脉侧支循环程度与HIF-1α表达水平呈正相关(r1=0.78,r2=0.84,均P<0.01). 结论 冠心病患者外周血单个核细胞HIF-1α表达水平与侧支循环形成明显相关,外周血单个核细胞HIF-1α水平的检测有利于预测冠心病患者侧支循环形成程度.
目的 探討冠心病患者體內單箇覈細胞缺氧誘導因子-1α(HIF-1α)錶達水平與側支循環建立程度的相關性. 方法 選擇冠狀動脈造影至少有一支血管狹窄超過70%的冠心病患者96例為冠心病組.其中42例有可見的側支循環,54例未見側支循環.另選冠狀動脈造影正常患者50例作為對照組.免疫組化和Western blot法檢測其外週血單箇覈細胞HIF-1α蛋白含量. 結果 免疫組化和Westem blot兩種方法檢測冠心病組HIF-1α錶達水平均明顯高于對照組(均P<0.01),冠心痛組中有側支循環患者比無側支循環患者HIF-1α錶達更多(均P<0.01),冠狀動脈側支循環程度與HIF-1α錶達水平呈正相關(r1=0.78,r2=0.84,均P<0.01). 結論 冠心病患者外週血單箇覈細胞HIF-1α錶達水平與側支循環形成明顯相關,外週血單箇覈細胞HIF-1α水平的檢測有利于預測冠心病患者側支循環形成程度.
목적 탐토관심병환자체내단개핵세포결양유도인자-1α(HIF-1α)표체수평여측지순배건립정도적상관성. 방법 선택관상동맥조영지소유일지혈관협착초과70%적관심병환자96례위관심병조.기중42례유가견적측지순배,54례미견측지순배.령선관상동맥조영정상환자50례작위대조조.면역조화화Western blot법검측기외주혈단개핵세포HIF-1α단백함량. 결과 면역조화화Westem blot량충방법검측관심병조HIF-1α표체수평균명현고우대조조(균P<0.01),관심통조중유측지순배환자비무측지순배환자HIF-1α표체경다(균P<0.01),관상동맥측지순배정도여HIF-1α표체수평정정상관(r1=0.78,r2=0.84,균P<0.01). 결론 관심병환자외주혈단개핵세포HIF-1α표체수평여측지순배형성명현상관,외주혈단개핵세포HIF-1α수평적검측유리우예측관심병환자측지순배형성정도.
Objective To investigate the relationship between hypoxia-inducible factor-1α(HIF-1α) and construction of collateral circulation in patients with coronary artery disease. Methods Collateral vessels were determined in 96 patients with≥70% narrowing of at least one coronary artery without prior revascularization,42 patients with coronary artery collaterals and 54 patients with no coronary artery collaterals.Another 50 cases with normal coronary arteries were selected as control.The levels of HIF-lα expression in monocytes and lymphocytes were tested by immunohistochemistry (IHC) and Western blot.Results Compared to the controls,the patients had higher expression of HIF-1α(P<0.01).Higher HIF-1α expression was found in patients with collaterals than in those without collaterals (P<0.01).Positive correlation was observed between the expression of HIF-1α protein and collateral score (r1=0.78,r2=0.84,P<0.01).Conclusion HIF-1α expression in circulating monocytes and lymphocytes are associated with collateral circulation.Detection HIF-1α might be helpful in the prognosis of patients with coronary artery disease.
