CAJ | 학술논문

目的探讨丙型肝炎病毒(HCV)感染对肾移植患者免疫功能的影响.方法提取患者外周血的淋巴细胞,应用碱性磷酸酶一抗碱性磷酸酶(APAAP)酶桥联法检测CD3+、CD4+、CD8+及CD16+细胞;免疫沉淀法测定血清中免疫球蛋白和补体水平.结果 HCV-IgG和/或HCV RNA阳性者(HCV阳性组)CD4+/CD8+值为0.90±0.25,显著低于HCV阴性组的1.46±0.54(P<0.05),主要是CD4+细胞下降显著;HCV阳性组CDl6+细胞高达27.8%,与HCV阴性组比较,差异有显著性(P＜0.01).HCV阳性组和阴性组的血清各免疫球蛋白和补体的差异无显著性(P＞0.05).结论 HCV阳性的肾移植患者其免疫功能紊乱.
목적 탐토병형간염병독(HCV)감염대신이식환자면역공능적영향.방법 제취환자외주혈적림파세포,응용감성린산매일항감성린산매(APAAP)매교련법검측CD3+、CD4+、CD8+급CD16+세포;면역침정법측정혈청중면역구단백화보체수평.결과 HCV-IgG화/혹HCV RNA양성자(HCV양성조)CD4+/CD8+치위0.90±0.25,현저저우HCV음성조적1.46±0.54(P<0.05),주요시CD4+세포하강현저;HCV양성조CDl6+세포고체27.8%,여HCV음성조비교,차이유현저성(P＜0.01).HCV양성조화음성조적혈청각면역구단백화보체적차이무현저성(P＞0.05).결론 HCV양성적신이식환자기면역공능문란.
Objectives To investigate the changes in CD4+/CD8+,NK cells,immunogrobulins and complements in the peripheral blood in the hepatitis C virus(HCV)positive patients undergoing kidney transplantation and their influence on the immune state.Methods APAAP test was used to determine the levels of CD3+,CD4+,CD8+ cells and natural killing(NK)cells in peripheral blood in the kidney graft recipients;meanwhile,serum immunoglobulins and complements were measured by immunoprecipitation method Results The mean ratio of CD4+/CD8+ was 0.90±0.25 in the HCV positive group.significantly lower than 1.46±0.54 in the HCV negative group(P＜0.05),accompanying with evident drop of the overall counts of CD4+ T cells but no remarkable changes in CD8+ T cells.The rate of CD16+cells(NK)accounted for 27.8%in the HCV positive group,higher than in the negative one(P＜0.01).There were no significant changes in serum immunoglobulins and complements between the two groups(P＞0.05).Conclusions The irnmune state in the HCV positive patients with kidney transplantation was too low to eliminate HCV efficiently.Determination of the CIN+/CD8+ and the rate of NK cells was helpful to the clinical treatment of the HCV positive recipients.