中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2011年
11期
1026-1030
,共5页
施晓雷%褚薛慧%张悦%韩冰%顾劲扬%肖江强%檀家俊%丁义涛
施曉雷%褚薛慧%張悅%韓冰%顧勁颺%肖江彊%檀傢俊%丁義濤
시효뢰%저설혜%장열%한빙%고경양%초강강%단가준%정의도
肝,人工%肝功能衰竭,急性%共培养%纳米膜支架
肝,人工%肝功能衰竭,急性%共培養%納米膜支架
간,인공%간공능쇠갈,급성%공배양%납미막지가
Liver,artificial%Liver failure,acute%Coculture%Nanofiber scaffold
目的 评价新型多层平板型生物人工肝治疗急性肝功能衰竭动物的疗效.方法 以新鲜猪肝细胞及猪骨髓基质干细胞为细胞来源,共培养于新型多层平板型生物反应器内,从而构建一种新型的生物人工肝.采用D-氨基半乳糖给药方式构建犬急性肝功能衰竭模型,实验组(n=8)给予生物人工肝治疗;对照组(n=8)仅给予一般监护.观察和检测所有动物一般情况、生化指标及生存率.结果 实验组动物经生物人工肝治疗后,肝性脑病及一般精神状况均得到较明显改善,丙氨酸氨基转移酶从( 1512±183) U/L降至(86±25) U/L;天冬氨酸氨基转移酶从(1472±365) U/L降至(46±1l)U/L;乳酸脱氢酶从(463±76) U/L降至(312±84)U/L;总胆红素从(28.8±6.2) μmol/L降至(12.5±3.6) μmol/L;血氨从(56±15)μmol/L降至(34±10) μmol/L,同时凝血功能及白蛋白水平亦得到改善.8条犬中,5条存活、3条死亡,治疗过程中未出现严重并发症.对照组动物一般情况未见明显改善,各项化验指标呈逐渐加重趋势,最终8条犬中5条死亡,3条存活.但两组生存率的差异无统计学意义(P=0.294).结论 新型多层平板型生物人工肝对急性肝功能衰竭动物具有显著疗效,是治疗急性肝功能衰竭的一种有效支持手段.
目的 評價新型多層平闆型生物人工肝治療急性肝功能衰竭動物的療效.方法 以新鮮豬肝細胞及豬骨髓基質榦細胞為細胞來源,共培養于新型多層平闆型生物反應器內,從而構建一種新型的生物人工肝.採用D-氨基半乳糖給藥方式構建犬急性肝功能衰竭模型,實驗組(n=8)給予生物人工肝治療;對照組(n=8)僅給予一般鑑護.觀察和檢測所有動物一般情況、生化指標及生存率.結果 實驗組動物經生物人工肝治療後,肝性腦病及一般精神狀況均得到較明顯改善,丙氨痠氨基轉移酶從( 1512±183) U/L降至(86±25) U/L;天鼕氨痠氨基轉移酶從(1472±365) U/L降至(46±1l)U/L;乳痠脫氫酶從(463±76) U/L降至(312±84)U/L;總膽紅素從(28.8±6.2) μmol/L降至(12.5±3.6) μmol/L;血氨從(56±15)μmol/L降至(34±10) μmol/L,同時凝血功能及白蛋白水平亦得到改善.8條犬中,5條存活、3條死亡,治療過程中未齣現嚴重併髮癥.對照組動物一般情況未見明顯改善,各項化驗指標呈逐漸加重趨勢,最終8條犬中5條死亡,3條存活.但兩組生存率的差異無統計學意義(P=0.294).結論 新型多層平闆型生物人工肝對急性肝功能衰竭動物具有顯著療效,是治療急性肝功能衰竭的一種有效支持手段.
목적 평개신형다층평판형생물인공간치료급성간공능쇠갈동물적료효.방법 이신선저간세포급저골수기질간세포위세포래원,공배양우신형다층평판형생물반응기내,종이구건일충신형적생물인공간.채용D-안기반유당급약방식구건견급성간공능쇠갈모형,실험조(n=8)급여생물인공간치료;대조조(n=8)부급여일반감호.관찰화검측소유동물일반정황、생화지표급생존솔.결과 실험조동물경생물인공간치료후,간성뇌병급일반정신상황균득도교명현개선,병안산안기전이매종( 1512±183) U/L강지(86±25) U/L;천동안산안기전이매종(1472±365) U/L강지(46±1l)U/L;유산탈경매종(463±76) U/L강지(312±84)U/L;총담홍소종(28.8±6.2) μmol/L강지(12.5±3.6) μmol/L;혈안종(56±15)μmol/L강지(34±10) μmol/L,동시응혈공능급백단백수평역득도개선.8조견중,5조존활、3조사망,치료과정중미출현엄중병발증.대조조동물일반정황미견명현개선,각항화험지표정축점가중추세,최종8조견중5조사망,3조존활.단량조생존솔적차이무통계학의의(P=0.294).결론 신형다층평판형생물인공간대급성간공능쇠갈동물구유현저료효,시치료급성간공능쇠갈적일충유효지지수단.
Objective To evaluate the efficacy of newly developed multi-layer flat-plate bioartificial liver in treatment of canines with acute liver failure.Methods Porcine hepatocytes and bone marrow mesenchymal stem cells were cocultured in newly developed multi-layer flat-plate bioreactor.Acute liver failure in canine models was induced by D-galactosamine administration.Sixteen canine models were divided into two groups:treatment group ( n =8 ) and control group ( n =8 ).Biochemical parameters were determined for 7 days after treatment and liver specimens were collected for histological analysis.Results Hepatic encephalopathy and general conditions were significantly improved in the treatment group,but no changes in the control group.Alanine aminotransferase was significantly decreased from ( 1512 ± 183 )U/L to ( 86 ± 25 ) U/L in the treatment group,aspartate aminotransferase was significantly decreased from ( 1472 ± 365) U/L to (46 ± 11 )U/L,lactate dehydrogenase was significantly decreased from (463 ± 76 )U/L to (312 ±84)U/L,total bilirubin was significantly decreased from ( 28.8 ± 6.2 ) μmol/L to ( 12.5 ± 3.6)μmol/L,ammonia was significantly decreased from (56 ± 15 ) μmol/L to (34 ± 10) μmol/L,and prothrombin time were significantly decreased in the treatment group but increased in the control group,albumin was improved in the treatment group but decreased in the control group.There were 5 canines survived in the treatment group but only 3 in the control group.But there was no difference on survival rates between the two group( P =0.294).Conclusion The application of newly developed multi-layer flat-plate bioartificial liver system was effective in the treatment of canines with acute liver failure.
