CAJ | 학술논문

目的观察神经轴突导向因子Netrin-1在人肝细胞癌(HCC)组织中的表达及其对肝癌细胞侵袭能力的影响.方法应用免疫组织化学SP方法检测45例肝癌手术标本癌旁及癌组织中Netrin-1的表达水平,用卡方检验分析其与临床病理参数的关系.在肝癌细胞SMMC-7721中转染Netrin-1基因,观测过表达Netrin-1肝癌细胞侵袭能力的改变.结果 Netrin-1在肝癌中的阳性表达率约为66.7%,主要在肝癌细胞胞质中表达,Netrin-1的阳性表达与性别、年龄、肿瘤大小以及细胞分化程度无关(P>0.05),『fii与门静脉痛栓以及淋巴转移有关(P<0.05).细胞侵袭实验结果显示,与实验对照组比较过表达Netrin-1显著促进肝癌细胞SMMC-7721侵袭能力(P<0.05).结论 HCC组织中高表达的Netrin-1与肿瘤侵袭转移有关,Netrin-1促进肝癌细胞侵袭能力可能是肝癌转移机制之一.
목적 관찰신경축돌도향인자Netrin-1재인간세포암(HCC)조직중적표체급기대간암세포침습능력적영향.방법 응용면역조직화학SP방법검측45례간암수술표본암방급암조직중Netrin-1적표체수평,용잡방검험분석기여림상병리삼수적관계.재간암세포SMMC-7721중전염Netrin-1기인,관측과표체Netrin-1간암세포침습능력적개변.결과 Netrin-1재간암중적양성표체솔약위66.7%,주요재간암세포포질중표체,Netrin-1적양성표체여성별、년령、종류대소이급세포분화정도무관(P>0.05),『fii여문정맥통전이급림파전이유관(P<0.05).세포침습실험결과현시,여실험대조조비교과표체Netrin-1현저촉진간암세포SMMC-7721침습능력(P<0.05).결론 HCC조직중고표체적Netrin-1여종류침습전이유관,Netrin-1촉진간암세포침습능력가능시간암전이궤제지일.
Objective To study Netrin-1 expression in hepatocellular carcinoma tissue and its effect on invasion of hepatocellular carcinoma cells. Methods The expression of Netrin-1 in 45 cases of hepatocellular carcinoma was detected by immunohistochemistry. The correlation between Netrin-1 and clinicopathologic features was analyzed by Chi-Squared test. The effect of Netrin-1 on invasion of SMMC- 7721 ceils was analyzed by invasion assay. Results The positive rate of Netrin-1 in HCC tissue was 66.7%. Netrin-1 expression was mainly located in kytoplasm of liver cancer cells. Netrin-1 expression was associated with lymph node metastasis, portal vein invasion, and metastatic lesions(P < 0.05 ), bet not with sex, age, tumor size, and cell differentiation (P >0.05 ). Cell invasion assay revealed that the number of migrating SMMC-7721 cells induced by Netrin-1 was increased(P < 0.05). Conclusion The up-regu- lation of Netrin-1 in HCC has a close relationship to the invasion of hepatoma cells. Invasion of hepatoma cells induced by Netrin-1 may be one of mechanisms of metastasis of HCC.