CAJ | 학술논문

目的探讨OAS-1基因SNP rs 10774671与慢性HBV感染者自发性HBeAg血清转换的关系.方法收集慢性HBV感染者HBeAg阳性组58例、anti-HBe阳性组68例和无HBV感染对照组72例的外周血,进行DNA提取扩增,再用竞争分化聚合酶链反应方法扩增,结合酶免疫显色检测该SNP的基因型,组间差异进行分析比较.结果 HBeAg阳性组的基因型GG+CA比例为31.0%、等位基因G频率为16.4%,而anti-HBe阳性组为48.5%、29.4%,对照组为50.0%、28.4%;HBeAg阳性组与anti-HBe阳性组或对照组之间的差异均有统计学意义,而anti-HBe阳性组与对照组之间的差异无统计学意义.结论 SNP rs 10774671等位基因G可能有助于慢性HBV感染者发生自发性HBeAg血清转换,其基因型检测对于慢性HBV感染者干扰素治疗有一定的预测价值.
목적 탐토OAS-1기인SNP rs 10774671여만성HBV감염자자발성HBeAg혈청전환적관계.방법 수집만성HBV감염자HBeAg양성조58례、anti-HBe양성조68례화무HBV감염대조조72례적외주혈,진행DNA제취확증,재용경쟁분화취합매련반응방법확증,결합매면역현색검측해SNP적기인형,조간차이진행분석비교.결과 HBeAg양성조적기인형GG+CA비례위31.0%、등위기인G빈솔위16.4%,이anti-HBe양성조위48.5%、29.4%,대조조위50.0%、28.4%;HBeAg양성조여anti-HBe양성조혹대조조지간적차이균유통계학의의,이anti-HBe양성조여대조조지간적차이무통계학의의.결론 SNP rs 10774671등위기인G가능유조우만성HBV감염자발생자발성HBeAg혈청전환,기기인형검측대우만성HBV감염자간우소치료유일정적예측개치.
Objective To investigate the relationship between the SNP rs10774671 on OAS-1 gene and spontaneous HBeAg seroconversion in chronic HBV infection. Methods Blood samples were collected from 58 HBeAg positive, 68 anti-HBe positive patients with chronic HBV infection, and 72 normal control cases without HBV infection. Chromosomal DNA was extracted and OAS-1 gene was amplified. SNP genotyping was performed with the competitively differentiated polymerase chain reaction and enzyme immunoassay. Results In HBeAg positive group, frequencies of genotype GG plus GA and allele G were 31.0% and 16.4%. They were 48.5% and 29.4% in anti-HBe positive group, and 50.0% and 28.4% in normal control group respectively. Differences between HBeAg positive group and anti-HBe positive group or normal control group were statistically significant. But they weren't between anti-HBe positive group and normal control group. Conclusion Allele G on SNP rs10774671 of OAS-1 gene maybe benefits patients with chronic HBV infection to achieve spontaneous HBeAg seroconversion. Genotyping on this SNP may be predicting valuable for interferon therapy for chronic HBV infection.