中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2008年
12期
1099-1102
,共4页
徐东兵%韩晓旭%张子宁%张曼%代娣%赵彬%王亚男%张晓丽%包名家%尚红
徐東兵%韓曉旭%張子寧%張曼%代娣%趙彬%王亞男%張曉麗%包名傢%尚紅
서동병%한효욱%장자저%장만%대제%조빈%왕아남%장효려%포명가%상홍
人免疫缺陷病毒-1%杀伤细胞免疫球蛋白样受体%聚合酶链反应-序列特异性引物技术
人免疫缺陷病毒-1%殺傷細胞免疫毬蛋白樣受體%聚閤酶鏈反應-序列特異性引物技術
인면역결함병독-1%살상세포면역구단백양수체%취합매련반응-서렬특이성인물기술
HIV-I%Killer cell Ig-like receptors%Polymerase chain reaction sequence-specific primers
目的 通过对中国HIV-1感染缓慢进展者(TP)和典型进展者(TP)杀伤细胞免疫球蛋白样受体(KIR)基因频率的研究,探讨中国人群K/R基因多态性与HIV-1感染者疾病进程的关系.方法 采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,分析43例HIV-1感染SP和38例TPKIR基因位点的多态性.结果 SP组和TP组的KIR2DS3等位基因频率分别为3.6%和14.2%,SP组显著低于TP组,两组的KIR2DS3等位基因频率差异具有统计学意义(P=0.018,OR=0.210,95%CI=0.053-0.833);SP组的活化性KIR基因数目低于TP组,但差异无统计学意义(P=0.208).结论 低表达KIR2D53基因可能是延缓中国HlV-1感染者疾病进程的保护因素之一.
目的 通過對中國HIV-1感染緩慢進展者(TP)和典型進展者(TP)殺傷細胞免疫毬蛋白樣受體(KIR)基因頻率的研究,探討中國人群K/R基因多態性與HIV-1感染者疾病進程的關繫.方法 採用聚閤酶鏈反應-序列特異性引物(PCR-SSP)技術,分析43例HIV-1感染SP和38例TPKIR基因位點的多態性.結果 SP組和TP組的KIR2DS3等位基因頻率分彆為3.6%和14.2%,SP組顯著低于TP組,兩組的KIR2DS3等位基因頻率差異具有統計學意義(P=0.018,OR=0.210,95%CI=0.053-0.833);SP組的活化性KIR基因數目低于TP組,但差異無統計學意義(P=0.208).結論 低錶達KIR2D53基因可能是延緩中國HlV-1感染者疾病進程的保護因素之一.
목적 통과대중국HIV-1감염완만진전자(TP)화전형진전자(TP)살상세포면역구단백양수체(KIR)기인빈솔적연구,탐토중국인군K/R기인다태성여HIV-1감염자질병진정적관계.방법 채용취합매련반응-서렬특이성인물(PCR-SSP)기술,분석43례HIV-1감염SP화38례TPKIR기인위점적다태성.결과 SP조화TP조적KIR2DS3등위기인빈솔분별위3.6%화14.2%,SP조현저저우TP조,량조적KIR2DS3등위기인빈솔차이구유통계학의의(P=0.018,OR=0.210,95%CI=0.053-0.833);SP조적활화성KIR기인수목저우TP조,단차이무통계학의의(P=0.208).결론 저표체KIR2D53기인가능시연완중국HlV-1감염자질병진정적보호인소지일.
Objective To investigate the killer cell lg-like receptors (KIR) gene frequency of HIV-1 infected slow progressors(SP) and typical progressors(TP), and to analyze the interaction between KIR alleles and the progression of HIV-1 infection in Chinese population. Methods Eighty-one HIV-1 posi-tive individuals including 43 SPs and 38 TPs were recruited. Carriage of KIR genes was assessed using poly-merase chain reaction sequence-specific primers (PCR-SSP) assays. Results KIR2DS3 gene frequency was significantly lower in SP group (3.6%) than that in TP group (14.2%), P =0. 018 ,OR =0. 210,95% CI =0.053-0.833. The number of activating KIR genes was less in SP group than that in TP group, but was not significant (P = 0. 208). Conclusion Lower KIR2DS3 gene frequency may potentially be associated with slower progression to AIDS in Chinese population.
