CAJ | 학술논문

目的探讨bcl-2反义寡核苷酸能否增加小细胞肺癌细胞NCI-H69的凋亡.方法将NCI-H69细胞培养传代,细胞共分4组,反义寡核苷酸组、正义寡核苷酸组、无义寡核苷酸组和空白对照组,通过脂质体将不同浓度的寡核苷酸导入细胞中,Western Blot法检测bcl-2蛋白的表达,流式细胞术检测细胞的凋亡率.结果与空白对照组比较,反义寡核苷酸组的bcl-2蛋白表达明显受到抑制,而正义寡核苷酸组和无义寡核苷酸组细胞的bcl-2蛋白表达则与空白对照组差异不明显.反义寡核苷酸组细胞在10、20、40μmol/L 3个不同浓度时的凋亡率分别是(9.97±1.54)%、(15.28±1.73)%、(21.41±1.85)%,明显高于空白组和正义链、无义链组阴性对照组,且差异有统计学意义(F=7.19～15.48,q=5.21～7.98,P＜0.01),而转染正义链和无义链组与空白组比较则差异无统计学意义.结论 bcl-2反义寡核苷酸能有效封闭bcl-2基因的表达,增加小细胞肺癌细胞的凋亡.
목적 탐토bcl-2반의과핵감산능부증가소세포폐암세포NCI-H69적조망.방법 장NCI-H69세포배양전대,세포공분4조,반의과핵감산조、정의과핵감산조、무의과핵감산조화공백대조조,통과지질체장불동농도적과핵감산도입세포중,Western Blot법검측bcl-2단백적표체,류식세포술검측세포적조망솔.결과 여공백대조조비교,반의과핵감산조적bcl-2단백표체명현수도억제,이정의과핵감산조화무의과핵감산조세포적bcl-2단백표체칙여공백대조조차이불명현.반의과핵감산조세포재10、20、40μmol/L 3개불동농도시적조망솔분별시(9.97±1.54)%、(15.28±1.73)%、(21.41±1.85)%,명현고우공백조화정의련、무의련조음성대조조,차차이유통계학의의(F=7.19～15.48,q=5.21～7.98,P＜0.01),이전염정의련화무의련조여공백조비교칙차이무통계학의의.결론 bcl-2반의과핵감산능유효봉폐bcl-2기인적표체,증가소세포폐암세포적조망.
Objective To study the effect of bcl-2 antisense oligodexynucleotides on the apoptosis in human small-cell lung cancer cell line NCI-H69. Methods Cultured cells were divided into 4 groups: antisense oligodexynucleotides(ASODN), sense oligodexynucleotides (SODN), nonsense oligodexynucleotides (NSODN) and control.The different bcl-2 oligodexynucleotides was transfected into corresponding cells using oligofectamine.The expression of bcl-2 was examined by Western blot.The apoptosis rates were measured by flow cytometry (FCM).Results The bcl-2 expression in ASODN group was significantly inhibited compared to the control group, SODN and NSODN groups, but it was not obviously inhibited in SODN and NSODN groups.The apoptosis rate of ASODN group in different concentration was (9.97±1.54) %, (15.28±1.73) % and (21.41±1.85) % respectively, it was significantly higher than that of the control group (F = 7.19-15.48,q = 5.21-7.98, P ＜0.01). Conclusion The bcl-2 ASODN could enhance cell apoptosis rate in small-cell lung cancer by blocking bcl-2 gene effectively.