CAJ | 학술논문

目的用粉尘螨(Dermatophagoides farnae)代谢培养基浸液(Dff)分离纯化粉尘螨2类变应原(Der f2)。方法采用Sephadex G-100层析、DEAE离子交换层析、PAGE等方法分离纯化Der f2，并对Der f2作 SDS-PAGE测定和热稳定性测定。结果从Dff分离纯化得到较纯的Der f2，经SDS-PAGE测定其相对分子质量为14 000，并经100℃加热15 min后，仍保留50％～83.3％生物学活性。结论从Dff中分离纯化的变应原，其物化性质符合Der f2特征。
목적용분진만(Dermatophagoides farnae)대사배양기침액(Dff)분리순화분진만2류변응원(Der f2)。방법채용Sephadex G-100층석、DEAE리자교환층석、PAGE등방법분리순화Der f2，병대Der f2작 SDS-PAGE측정화열은정성측정。결과종Dff분리순화득도교순적Der f2，경SDS-PAGE측정기상대분자질 량위14 000，병경100℃가열15 min후，잉보류50％～83.3％생물학활성。결론종Dff중분리순화적변응원， 기물화성질부합Der f2특정。
Purpose To purify Der f2 allergen from the spent culture medium extract of Dermatophagoides farinae mite(Dff). Methods We purified Der f2 with the aid of Sephadex G-100 gel filtration, DEAE-ion exchange chromatography and polyacrylamide gel electrophoresis, detected the molecular weight of Der f2 using SDS-PAGE method,and analysed the thermostability of Der f2 by heating at 100℃ for 15 min. Results The pure Der f2 was obtained from Dff. The molecular weight of Der f2 was 14 000.The heated-treated Der f2 retained 50% ～83.3% of the activity of the native allergen. Conclusions The properties of purified allergen coincided with the characterization of Der f2.