CAJ | 학술논문

目的使用不同饲料配方及喂饲方法制备具有不同胰岛素敏感性(IS)的大鼠模型方法132只雄性SD大鼠分为3组(对照组、肥胖组、限食组),使用两种饲料(基础与高脂高能)及两种喂饲方式(自由与限食)喂饲6个月.各组大鼠在喂饲至2个月、4个月、6个月时采用正常血糖、高血浆胰岛素钳夹技术检测IS并进行口服糖耐量试验;同时检测大鼠体重、内脏体脂重量及空腹血浆胰岛素(FIns)水平.结果在此喂饲条件下,各组大鼠IS由高到低依次为:限食组＞对照组＞肥胖组.肥胖组大鼠喂饲2个月时就已出现IS明显减退(55%)和糖耐量受损,并随喂饲时间延长而加重;对照组大鼠喂饲2个月、4个月时IS与糖耐量结果正常,6个月时糖耐量与IS有所减退;限食组大鼠始终保持胰岛素高敏状态,实验结束时,该组大鼠IS分别为对照组和肥胖组的5倍与10倍.三组大鼠的体重、体内脂肪含量及血浆胰岛素水平均有相应的变化.结论本实验采用的不同饲料及喂饲方式可以成功地制备稳定可靠的、具有不同IS的大鼠模型.
목적사용불동사료배방급위사방법제비구유불동이도소민감성(IS)적대서모형방법132지웅성SD대서분위3조(대조조、비반조、한식조),사용량충사료(기출여고지고능)급량충위사방식(자유여한식)위사6개월.각조대서재위사지2개월、4개월、6개월시채용정상혈당、고혈장이도소겸협기술검측IS병진행구복당내량시험;동시검측대서체중、내장체지중량급공복혈장이도소(FIns)수평.결과재차위사조건하,각조대서IS유고도저의차위:한식조＞대조조＞비반조.비반조대서위사2개월시취이출현IS명현감퇴(55%)화당내량수손,병수위사시간연장이가중;대조조대서위사2개월、4개월시IS여당내량결과정상,6개월시당내량여IS유소감퇴;한식조대서시종보지이도소고민상태,실험결속시,해조대서IS분별위대조조화비반조적5배여10배.삼조대서적체중、체내지방함량급혈장이도소수평균유상응적변화.결론본실험채용적불동사료급위사방식가이성공지제비은정가고적、구유불동IS적대서모형.
Objective Preparing of a rat model for study of mechanismand process of insulin re-sistance by different diet composition and caloric restriction. Methods 132 rats were divided into 3groups :control , obesity and caloric restriction. Euglycemic-hyperinsulinemic clamp technique andOGTT were taken every two months . At the same ti me ,plasma and tissue samples and body weightwere taken fromeach group .Insulinlevels and fat tissue mass were determined . Results The orderof insulin sensitivity and glucose tolerance ability were caloric restriction >control >obesity group .The order of plasma insulin level was obesity >control >caloric restriction groups .The body weightand visceral fat mass wereincreased significantlyin obesity group . Conclusions The protocols of di-ets andfeeding patterns adoptedinthis study are able toinduce a rat model with different insulin sensi-tivity .The accumulation of body fat mass is related to the metabolismabnormalities in vivo .