CAJ | 학술논문

通过逆转录PCR(RT-PCR及快速扩增cDNA末端(RACE)技术从中国白梨品种'雪花'梨中克隆到S16-RNase基因的全长cDNA序列(GenBank接受号为DQ991388)该cDNA克隆总长1101 bp,包含1个完整的开放阅读框,编码228个氨基酸S16-RNase表现出梨S-RNase基因的基本结构特征,即其具有5个保守区(C1,C2,C3,RC4及C5)和1个高变区在推导氨基酸水平上,S16-RNase与梨其他S-RNase基因的相似性为63%至74%,但与苹果S9-RNase的相似性高达95%通过多序列比对构建进化树,分析梨S16-RNase与蔷薇科植物其他S-RNase基因的遗传进化关系结果表明,S16-RNase与苹果亚科S-RNase基因形成一个亚科特异而非种特异的S-RNase基因类群;且不同S-RNase基因间存在属内种间遗传距离远于属间种问遗传距离现象
통과역전록PCR(RT-PCR급쾌속확증cDNA말단(RACE)기술종중국백리품충'설화'리중극륭도S16-RNase기인적전장cDNA서렬(GenBank접수호위DQ991388)해cDNA극륭총장1101 bp,포함1개완정적개방열독광,편마228개안기산S16-RNase표현출리S-RNase기인적기본결구특정,즉기구유5개보수구(C1,C2,C3,RC4급C5)화1개고변구재추도안기산수평상,S16-RNase여리기타S-RNase기인적상사성위63%지74%,단여평과S9-RNase적상사성고체95%통과다서렬비대구건진화수,분석리S16-RNase여장미과식물기타S-RNase기인적유전진화관계결과표명,S16-RNase여평과아과S-RNase기인형성일개아과특이이비충특이적S-RNase기인류군;차불동S-RNase기인간존재속내충간유전거리원우속간충문유전거리현상
The full length Edna encoding S16-Rnase(GenBank accession no.DQ991388)was cloned from styles of Chinese white pear(Pyrus bretschneideri Rehd.)cultivar'Xuehua'by reverse transcription-PCR(RT-PCR)and rapid amplification of Cdna ends(RACE)technology.The S16-Rnase Cdna contained 1101 nucleotides including a complete open reading frame(ORF)predicted to encode a protein of 228 amino acids.The S16-Rnase displayed the basic structural features of pear S Rnases.I.e.five conserved regions(C1,C2,C3,RC4 and C5)and a hypervariable(HV)region.At the deduced amino acid level,S16-Rnase showed 63%to 74%similarity with other pear S-Rnases,whereas it showed an extremely high similarity(95%)with apple(Malusdomestica Borkh.)S9-Rnase.Based on multiple amino acid sequences alignment and phylogenetic tree construction,the evolutionary relationship between S16-nase and other S-Rnases of Rosaceae plants was investigated.Results show that S6-Rnase clustered with maloideous S-Rnases,forming a subfamily-specific S-Rnase group but not a species-specific group.In addition,the genetic distance of S-Rnases was greater among some intragenus species than that of among intergenus species.