CAJ | 학술논문

[目的]为优质稻米种苗培育及种质资源保护提供参考.[方法]以曲阜香稻新品系103的成熟种子为材料,取其成熟胚接种在MS1培养基中诱导胚性愈伤组织,选取松脆性胚性愈伤组织建立悬浮细胞系.[结果]初始愈伤组织呈深黄色、瘤状、质地紧密、生长旺盛、易分化出芽,但难以进行细胞悬浮培养;在MS2培养基中继代培养120 d后,愈伤组织呈浅黄色、颗粒状、松脆型胚性愈伤组织;松脆型胚性愈伤组织在悬浮培养基中继代培养2～3次后,不断释放出小细胞团和单细胞,用300目不锈钢网对培养液进行过滤,滤液培养120 d可建立起浅黄色、澄清、均一的胚性悬浮细胞系.[结论]建立了分散性好、生长快的水稻胚性悬浮细胞系.
[목적]위우질도미충묘배육급충질자원보호제공삼고.[방법]이곡부향도신품계103적성숙충자위재료,취기성숙배접충재MS1배양기중유도배성유상조직,선취송취성배성유상조직건립현부세포계.[결과]초시유상조직정심황색、류상、질지긴밀、생장왕성、역분화출아,단난이진행세포현부배양;재MS2배양기중계대배양120 d후,유상조직정천황색、과립상、송취형배성유상조직;송취형배성유상조직재현부배양기중계대배양2～3차후,불단석방출소세포단화단세포,용300목불수강망대배양액진행과려,려액배양120 d가건립기천황색、징청、균일적배성현부세포계.[결론]건립료분산성호、생장쾌적수도배성현부세포계.
[Objective] The study was to provide the reference for seedling fostering of rice with high quality and protection of its germplasm.[Method] With grains of new rice line 103 from Qvfu Xiangdao as the materials, their mature embryo were inoculated in MS1 medium to induce embryogenic callus, and then the crisp embryogenic callus were collected to establish suspension cell.[Result] The initial tissues showed deeply yellow, warty with close texture and exuberant growth, they could easily differentiate bud, but were not suitable for cell suspension culture.After 120 d of subculture in MS2 medium, the callus became to light yellow and granular crisp embryogenic callus.When crisp embryogenic callus were cultured for 2-3 times in suspension medium, the small cell clusters and single cell was released out continuously.The culture medium was leached for culture with 300 mesh stainless steel net, and after 120 d of culture, the light yellow, clear and homogenous embryogenic cell suspension line was established.[Conclusion] The study established the embryogenic cell suspension line of rice with good suspension and fast growth.