癌症
癌癥
암증
CHINESE JOURNAL OF CANCER
2009年
11期
1158-1162
,共5页
姜智%陈宝安%吴强%夏国华%张宇%高峰%洪铁艳%徐翠荣%程坚%李国宏%陈文姬%刘莉洁%李小毛%王雪梅
薑智%陳寶安%吳彊%夏國華%張宇%高峰%洪鐵豔%徐翠榮%程堅%李國宏%陳文姬%劉莉潔%李小毛%王雪梅
강지%진보안%오강%하국화%장우%고봉%홍철염%서취영%정견%리국굉%진문희%류리길%리소모%왕설매
磁性纳米Fe_3O_4颗粒%卵巢肿瘤%SKOV3/DDP细胞%耐药%逆转%凋亡相关基因
磁性納米Fe_3O_4顆粒%卵巢腫瘤%SKOV3/DDP細胞%耐藥%逆轉%凋亡相關基因
자성납미Fe_3O_4과립%란소종류%SKOV3/DDP세포%내약%역전%조망상관기인
Fe_3O_4-magnetic nanoparticles%ovarian neoplasm%SKOV3/DDP cells%multi-drug resistance%reverse%apoptosis-associated genes
背景与目的:目前认为多药耐药(multi-drug resistance,MDR)是卵巢癌化疗失败和复发的主要原因.本研究探讨磁性纳米Fe_3O_4颗粒对卵巢癌细胞(SKOV3/DDP)耐药性的逆转作用及其与凋亡相关基因的关系.方法:将卵巢癌细胞的耐药株SKOV3/DDP分为对照组、顺铂组(DDP组)、磁性纳米Fe_3O_4颗粒组(Fe_3O_4-MNPs组)、DDP+磁性纳米Fe_3O_4颗粒组(DDP+Fe_3O_4-MNPs组)4个组,用MTT比色法检测对细胞增殖的抑制作用,流式细胞术测定细胞凋亡,电感耦合等离子体原子发射光谱法测细胞内顺铂的含量,RT-PCR检测凋亡相关基因Bcl-2和Survivin mRNA在SKOV3/DDP中的表达.结果:Fe_3O_4-MNPs组细胞耐药性的逆转倍数为2.259.DDP+Fe_3O_4-MNPs组细胞凋亡率[(42.1±5.6)%vs.(26.9±4.1)%]及细胞内铂含量[(0.074±0.006)μmol/L vs.(0.057±0.003)μmol/L]均高于DDP组(P＜0.05),其差异有统计学意义(P＜0.05).DDP+Fe_3O_4-MNPs组细胞bcl-2、survivin mRNA表达均低于DDP组,其差异有统计学意义(P＜0.05).结论:磁性纳米Fe_3O_4颗粒可逆转卵巢癌细胞对顺铂的耐受性,作用机制可能与其降低抗凋亡基因bcl-2和survivin mRNA表达有关.
揹景與目的:目前認為多藥耐藥(multi-drug resistance,MDR)是卵巢癌化療失敗和複髮的主要原因.本研究探討磁性納米Fe_3O_4顆粒對卵巢癌細胞(SKOV3/DDP)耐藥性的逆轉作用及其與凋亡相關基因的關繫.方法:將卵巢癌細胞的耐藥株SKOV3/DDP分為對照組、順鉑組(DDP組)、磁性納米Fe_3O_4顆粒組(Fe_3O_4-MNPs組)、DDP+磁性納米Fe_3O_4顆粒組(DDP+Fe_3O_4-MNPs組)4箇組,用MTT比色法檢測對細胞增殖的抑製作用,流式細胞術測定細胞凋亡,電感耦閤等離子體原子髮射光譜法測細胞內順鉑的含量,RT-PCR檢測凋亡相關基因Bcl-2和Survivin mRNA在SKOV3/DDP中的錶達.結果:Fe_3O_4-MNPs組細胞耐藥性的逆轉倍數為2.259.DDP+Fe_3O_4-MNPs組細胞凋亡率[(42.1±5.6)%vs.(26.9±4.1)%]及細胞內鉑含量[(0.074±0.006)μmol/L vs.(0.057±0.003)μmol/L]均高于DDP組(P＜0.05),其差異有統計學意義(P＜0.05).DDP+Fe_3O_4-MNPs組細胞bcl-2、survivin mRNA錶達均低于DDP組,其差異有統計學意義(P＜0.05).結論:磁性納米Fe_3O_4顆粒可逆轉卵巢癌細胞對順鉑的耐受性,作用機製可能與其降低抗凋亡基因bcl-2和survivin mRNA錶達有關.
배경여목적:목전인위다약내약(multi-drug resistance,MDR)시란소암화료실패화복발적주요원인.본연구탐토자성납미Fe_3O_4과립대란소암세포(SKOV3/DDP)내약성적역전작용급기여조망상관기인적관계.방법:장란소암세포적내약주SKOV3/DDP분위대조조、순박조(DDP조)、자성납미Fe_3O_4과립조(Fe_3O_4-MNPs조)、DDP+자성납미Fe_3O_4과립조(DDP+Fe_3O_4-MNPs조)4개조,용MTT비색법검측대세포증식적억제작용,류식세포술측정세포조망,전감우합등리자체원자발사광보법측세포내순박적함량,RT-PCR검측조망상관기인Bcl-2화Survivin mRNA재SKOV3/DDP중적표체.결과:Fe_3O_4-MNPs조세포내약성적역전배수위2.259.DDP+Fe_3O_4-MNPs조세포조망솔[(42.1±5.6)%vs.(26.9±4.1)%]급세포내박함량[(0.074±0.006)μmol/L vs.(0.057±0.003)μmol/L]균고우DDP조(P＜0.05),기차이유통계학의의(P＜0.05).DDP+Fe_3O_4-MNPs조세포bcl-2、survivin mRNA표체균저우DDP조,기차이유통계학의의(P＜0.05).결론:자성납미Fe_3O_4과립가역전란소암세포대순박적내수성,작용궤제가능여기강저항조망기인bcl-2화survivin mRNA표체유관.
Background and Objective: Resistance to cisplatin (DDP)remains a major obstacle for the successful treatment of ovarian cancer.This study was to determine the reversal effect of Fe_3O_4-magnetic nanoparticles (MNPs)on DDP-resistance of ovarian carcinoma cell line SKOV3/DDP,and to explore its correlation with apoptosis-associated genes.Methods: SKOV3/DDP cells were divided into the DDP group,the Fe_3O_4-MNPs group, the combination (DDP plus Fe_3O_4-MNPs) group,and the control group. Cell proliferation was determined by MTT assay.Cell apoptosis was analyzed by flow cytometry (FCM).Intracellular DDP level was detected by inductively coupled plasma atomic emission spectrometry(ICP-AES).The expressions of apoptosis-associated genes, bcl-2, and survivin were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results:Fe_3O_4-MNPs reversed DDPresistance of SKOV3/DDP cells by 2.259 folds.The cell apotosis rate and the intracellular DDP level were significantly higher in the combination group than in the DDP group (P＜0.05). Moreover,the mRNA levels of bcl-2 and survivin were significantly lower in the combination group than in the DDP group (P＜0.05). Conclusions:Fe_3O_4-MNPs can reverse the DDP resistance of ovarian carcinoma SKOV3/DDP cells, and the effect may be ascribed to the down-regulation of bcl-2 and survivin expression.
