CAJ | 학술논문

本研究采集了不同剂量曲古菌素A(TSA)分别处埋24h和48h下的人宫颈癌细胞株(HeLa cell)的培养基RPMI1640的紫外吸收光谱.结果显示:细胞培养基分别在233nm和275nm处有特征性的吸收峰.数据的进一步分析发现:不同剂量的TSA在处理细胞时间一致时,培养基的吸收光谱存在明显的差别.即:1)当TSA处理细胞24小时,伴随着用药剂量的增大,细胞培养基在233nm处的吸收值先降后升,275nm处的吸收值同样先降后升:2)当TSA处理细胞48小时,伴随着用药剂量的增大,细胞培养基在233nm处的吸收值越来越低,275nm处的吸收值却越来越高.这一结果反映了培养基中芳香族氨基酸和半胱氨酸残基的含量及比例变化,其变化规律与药物引起细胞的增殖改变有着密切的联系,并为动物实验和临床应用奠定了基础.
본연구채집료불동제량곡고균소A(TSA)분별처매24h화48h하적인궁경암세포주(HeLa cell)적배양기RPMI1640적자외흡수광보.결과현시:세포배양기분별재233nm화275nm처유특정성적흡수봉.수거적진일보분석발현:불동제량적TSA재처리세포시간일치시,배양기적흡수광보존재명현적차별.즉:1)당TSA처리세포24소시,반수착용약제량적증대,세포배양기재233nm처적흡수치선강후승,275nm처적흡수치동양선강후승:2)당TSA처리세포48소시,반수착용약제량적증대,세포배양기재233nm처적흡수치월래월저,275nm처적흡수치각월래월고.저일결과반영료배양기중방향족안기산화반광안산잔기적함량급비례변화,기변화규률여약물인기세포적증식개변유착밀절적련계,병위동물실험화림상응용전정료기출.
The 1640 culture medium for HeLa cell has been studied after treated by Trichostain A(TSA)for 24 or 48h.The results showed that there was characteristic absorption peak in culture medium at 233nm and 275nm wavelength.Those results were furthef analyzed indieated there was obvious difference in the absorption spectrum of culture medium When HeLa cells were treated bv TSA with different doses at the same time.1)When HeLa cells were treated for 24h by TSA,the absorbance was down firstly,then up at 233nm wavelength with the dose increasing.In addition,the same trend occurred at 275nm wavelength.2)When HeLa cells were treated for 48h by TSA,the absorbance was less and less gradually at 233nm wavelength with the dose increasing.However,the absorbance was higher and higher at 275nm wavelength.Those results indicate there was change in the residues content and the ratio of aromatic Amino Acids and Crsteine,which was closely linked to change in drug-indueed cell proliferation.This study laid the foundation foranimal experiment and clinical application.