CAJ | 학술논문

鉴于猪瘟病毒不能使家兔发病,但能使之产生免疫原性,而且猪瘟兔化弱度苗能在兔体的定型热反应.本研究选用家兔为试验模型,将自制的亚单位疫苗用弗氏佐剂乳化后免疫家兔,持续一定的免疫周期后,用猪瘟活疫苗(Ⅱ)攻击免疫及对照试验家兔.根据T淋巴细胞增殖试验、阻断ELISA及攻毒保护试验对所制备的抗原进行了全面的评价.结果表明,该免疫原具有良好的免疫原性,而且能保护免疫家兔为4/4.可以进行下一步的开发和利用价值.以期该疫苗能为猪瘟的防治做出贡献.
감우저온병독불능사가토발병,단능사지산생면역원성,이차저온토화약도묘능재토체적정형열반응.본연구선용가토위시험모형,장자제적아단위역묘용불씨좌제유화후면역가토,지속일정적면역주기후,용저온활역묘(Ⅱ)공격면역급대조시험가토.근거T림파세포증식시험、조단ELISA급공독보호시험대소제비적항원진행료전면적평개.결과표명,해면역원구유량호적면역원성,이차능보호면역가토위4/4.가이진행하일보적개발화이용개치.이기해역묘능위저온적방치주출공헌.
To investigate the immunogeneicity of a subunit vaccine of E2 glycoprotein of classical swine fever virus(CSFV). In this study, the rabbits were immunized with the homemade antigen (recombinant E2 protein) ,Tlymphocyte proliferation response, blocking ELISA and virus attacked test were used to detect the effect of the immunized responses in guinea pigs. The results indicated that a retroviral based vaccine carrying the E2 gene of CSF was able to elicit strong CSFV specific humoral immune responses in guinea pigs. These results encourages further work towards the development of a vaccine against CSFV infection.