中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
10期
1843-1846
,共4页
罗宏武%黄湘俊%刘浔阳%黄飞舟
囉宏武%黃湘俊%劉潯暘%黃飛舟
라굉무%황상준%류심양%황비주
腺相关病毒%绿色荧光蛋白%基因%转染%人羊膜上皮细胞
腺相關病毒%綠色熒光蛋白%基因%轉染%人羊膜上皮細胞
선상관병독%록색형광단백%기인%전염%인양막상피세포
背景:人羊膜上皮细胞易于获得,采集简单,是细胞移植和组织修复的理想种子细胞,目前国内外尚无关于标记、示踪体外培养的人羊膜上皮细胞的报道.目的:探讨腺相关病毒载体介导绿色荧光蛋白摹因对体外培养的人羊膜上皮细胞的转染效果.方法:取人羊膜标本,以胰蛋白酶消化法分离培养人羊膜上皮细胞,采用含绿色荧光蛋白的腺相关病毒进行转染,检测其转染效率.结果与结论:人羊膜上皮细胞可成功地在体外进行原代和传代培养,经含绿色荧光蛋白的腺相关病毒颗粒转染后,可稳定高效表达绿色荧光蛋白,转染效率达58%.
揹景:人羊膜上皮細胞易于穫得,採集簡單,是細胞移植和組織脩複的理想種子細胞,目前國內外尚無關于標記、示蹤體外培養的人羊膜上皮細胞的報道.目的:探討腺相關病毒載體介導綠色熒光蛋白摹因對體外培養的人羊膜上皮細胞的轉染效果.方法:取人羊膜標本,以胰蛋白酶消化法分離培養人羊膜上皮細胞,採用含綠色熒光蛋白的腺相關病毒進行轉染,檢測其轉染效率.結果與結論:人羊膜上皮細胞可成功地在體外進行原代和傳代培養,經含綠色熒光蛋白的腺相關病毒顆粒轉染後,可穩定高效錶達綠色熒光蛋白,轉染效率達58%.
배경:인양막상피세포역우획득,채집간단,시세포이식화조직수복적이상충자세포,목전국내외상무관우표기、시종체외배양적인양막상피세포적보도.목적:탐토선상관병독재체개도록색형광단백모인대체외배양적인양막상피세포적전염효과.방법:취인양막표본,이이단백매소화법분리배양인양막상피세포,채용함록색형광단백적선상관병독진행전염,검측기전염효솔.결과여결론:인양막상피세포가성공지재체외진행원대화전대배양,경함록색형광단백적선상관병독과립전염후,가은정고효표체록색형광단백,전염효솔체58%.
BACKGROUND:Human amniotic epithelial cells(AECs)are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair.Currently,marking and tracing of human AECs remains rarely reported.OBJECTIVE:To explore the efficiency of adeno-associated virus(AAV)vector-medicated green fluorescent protein(GFP)on in vitro cultured human AECs transfection.METHODS:Human amnion samples were harvested and trypsinized to isolate human AECs.The AECs were transfected with AAV-GFP,and the transfection efficiency was detected.RESULTS AND CONCLUSION:Human AECs were successfully primary cultured and passaged in vitro.AAV-GFP-transfected AECs stably and highly expressed GFP,with a transfection efficiency of 58%.