CAJ | 학술논문

背景:高脂饮食能引起肥胖,成年人肥胖能增加骨密度,对健康有一定的正面作用,而高脂饮食对生长快速的儿童骨骼发育的影响并不十分明确.目的:观察高脂饮食对雌性幼鼠骨胳发育的影响.方法:取12只4周龄雌性CD1小鼠,分别给予高脂饮食和正常饮食,喂养10周后用双能X射线骨密度仪扫描全身;用三点弯曲实验检测骨生物力学特征;用酶联免疫分析法检测血清中骨转换标志物;股骨组织切片苏木精-伊红染色观察骨小梁变化和骨髓的脂肪化程度.结果与结论:高脂饮食组小鼠的体质量、体脂含量均显著高于正常饮食组,但全身的骨密度、骨矿物质含量、骨面积和肌肉组织含量与正常饮食组无显著差异,但腰椎的骨密度、骨矿物质含量和骨面积都显著低于正常饮食组,而股骨的骨密度、骨矿物质含量和骨面积都显著高于正常饮食组,经体质量或体脂含量校正后虽然无显著统计学差异,但高脂饮食组全身和股骨的骨密度、骨矿物质含量和骨面积都呈现了低于正常饮食组的趋势;两组在骨生物力学特性方面的比较没有显著差异;高脂饮食组的血清骨转换标志物浓度较正常饮食组低;组织切片可见高脂饮食组的骨髓腔中有大量脂肪浸润和骨小梁宽度和面积减小.提示肥胖对生长旺盛阶段的幼鼠骨骼发育有不良影响,椎骨的骨矿物化程度下降,承重部位骨量的增加不能充分代偿体质量的增加.
배경:고지음식능인기비반,성년인비반능증가골밀도,대건강유일정적정면작용,이고지음식대생장쾌속적인동골격발육적영향병불십분명학.목적:관찰고지음식대자성유서골각발육적영향.방법:취12지4주령자성CD1소서,분별급여고지음식화정상음식,위양10주후용쌍능X사선골밀도의소묘전신;용삼점만곡실험검측골생물역학특정;용매련면역분석법검측혈청중골전환표지물;고골조직절편소목정-이홍염색관찰골소량변화화골수적지방화정도.결과여결론:고지음식조소서적체질량、체지함량균현저고우정상음식조,단전신적골밀도、골광물질함량、골면적화기육조직함량여정상음식조무현저차이,단요추적골밀도、골광물질함량화골면적도현저저우정상음식조,이고골적골밀도、골광물질함량화골면적도현저고우정상음식조,경체질량혹체지함량교정후수연무현저통계학차이,단고지음식조전신화고골적골밀도、골광물질함량화골면적도정현료저우정상음식조적추세;량조재골생물역학특성방면적비교몰유현저차이;고지음식조적혈청골전환표지물농도교정상음식조저;조직절편가견고지음식조적골수강중유대량지방침윤화골소량관도화면적감소.제시비반대생장왕성계단적유서골격발육유불량영향,추골적골광물화정도하강,승중부위골량적증가불능충분대상체질량적증가.
BACKGROUND:High fat diet (HFD) can induce overweight and obesity,which have been considered to positively affect bone mineral density (BMD) in adults.However,it is unclear how HFD affects the bone development during childhood.OBJECTIVE:To determine the effect of HFD on bone development in young female mice.METHODS:Twelve female CD1 mice were fed with either HFD or normal fat diet (NFD) starting at 4-week of age for 10 weeks.The bone mineral content (BMC),BMD,fat and lean mass were examined in 14-week old mice using dual-energy X-ray absorptiometry,and bone biomechanical properties were also evaluated using three-point bending test.Serum concentration of bone metabolic markers was measured using enzyme immunoassay.Femora were sectioned in the transverse plane and stained with hematoxylin and eosin for observing the adiposity of bone marrow and changes in trabecular bone area.RESULTS AND CONCLUSION:The body weight and fat mass in HFD-treated mice were increased compared with those in NFD-treated mice,respectively.There were no significant differences between HFD-treated and NFD-treated mice in whole body BMD,BMC,bone area and lean mass.However,the spine BMC and bone area in HFD mice were significantly lower than that in NFD mice,while femoral BMD,BMC and bone area in HFD mice were significantly greater than that in NFD mice.But,there was no statistically different in bone biomechanical values between the two groups.Bone metabolic markers were lower in HFD mice than NFD mice,indicating the less active of bone metabolism in HFD mice.It is suggested that HFD can produce deleterious effect on bone during the active growing phase of young mice.Vertebral bone is more sensitive to this negative effect than cortical bone due to the decreased vertebral mineralization.Weight-bearing bone does not response sufficiently to compensate for the excessive weight gaining.