中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2012年
3期
275-278
,共4页
巫姜%李涛%张志%赵晋明%王俊华%张雪%林仁勇%温浩
巫薑%李濤%張誌%趙晉明%王俊華%張雪%林仁勇%溫浩
무강%리도%장지%조진명%왕준화%장설%림인용%온호
细粒棘球蚴病%程序性死亡受体配体1%流式细胞术%免疫组织化学法
細粒棘毬蚴病%程序性死亡受體配體1%流式細胞術%免疫組織化學法
세립극구유병%정서성사망수체배체1%류식세포술%면역조직화학법
Echinococcosis%Programmed death receptor ligand 1%Flow cytometry%Immunocytochemistry
目的 探讨肝囊型包虫病患者外周血单个核细胞(PBMC)表面程序性死亡受体配体1(PD-L1)的表达及其与血清1FN-γ水平之间的关系.方法 回顾性分析2010年6月至2011年2月新疆医科大学第一附属医院收治的63例肝囊型包虫病患者的临床资料.根据世界卫生组织包虫病专家组包虫病标准化分型原则将患者分为肝包虫活性组(38例)和肝包虫无活性组(25例),20例肝血管瘤患者及健康志愿者作为正常对照组.采用流式细胞仪及免疫组织化学法检测PD-L1阳性表达率,ELISA法检测血清IFN-γ的表达水平.组间比较采用成组设计资料£检验和单因素方差分析,进一步两两比较采用LSD检验,总体分布采用x2检验,PD-LI阳性表达率和IFN-γ的表达水平的相关性分析采用Pearson检验.结果 流式细胞仪检测肝包虫活性组、肝包虫无活性组和正常对照组PBMC表面PD-L1的阳性表达率分别为12.1%士3.8%、10.9%士2.5%和9.1%±2.5%,肝包虫活性组与正常对照组PD-L1的阳性表达率比较,差异有统计学意义(t =3.327,P<0.05).免疫组织化学检测肝包虫活性组、肝包虫无活性组和正常对照组PBMC涂片中PD-L1的阳性表达率分别为11.9%±3.4%、10.6%±2.9%和9.5%±3.6%,肝包虫活性组与正常对照组PD-L1的阳性表达率比较,差异有统计学意义(t=2.470,P<0.05).肝包虫活性组、肝包虫无活性组和正常对照组血清IFN-γ表达分别为(141±38) μg/L、(124±32) μg/L和(105±42) μg/L,肝包虫活性组与正常对照组IFN-γ表达水平比较,差异有统计学意义(t=3.280,P<0.05).流式细胞仪和免疫组织化学检测PBMC的PD-L1阳性表达率与血清IFN-γ表达水平均呈明显正相关(r=0.59,0.61,P<0.05).结论 PD-L1可能在IFN-γ的作用下发挥着促进肝包虫免疫逃避的重要作用.
目的 探討肝囊型包蟲病患者外週血單箇覈細胞(PBMC)錶麵程序性死亡受體配體1(PD-L1)的錶達及其與血清1FN-γ水平之間的關繫.方法 迴顧性分析2010年6月至2011年2月新疆醫科大學第一附屬醫院收治的63例肝囊型包蟲病患者的臨床資料.根據世界衛生組織包蟲病專傢組包蟲病標準化分型原則將患者分為肝包蟲活性組(38例)和肝包蟲無活性組(25例),20例肝血管瘤患者及健康誌願者作為正常對照組.採用流式細胞儀及免疫組織化學法檢測PD-L1暘性錶達率,ELISA法檢測血清IFN-γ的錶達水平.組間比較採用成組設計資料£檢驗和單因素方差分析,進一步兩兩比較採用LSD檢驗,總體分佈採用x2檢驗,PD-LI暘性錶達率和IFN-γ的錶達水平的相關性分析採用Pearson檢驗.結果 流式細胞儀檢測肝包蟲活性組、肝包蟲無活性組和正常對照組PBMC錶麵PD-L1的暘性錶達率分彆為12.1%士3.8%、10.9%士2.5%和9.1%±2.5%,肝包蟲活性組與正常對照組PD-L1的暘性錶達率比較,差異有統計學意義(t =3.327,P<0.05).免疫組織化學檢測肝包蟲活性組、肝包蟲無活性組和正常對照組PBMC塗片中PD-L1的暘性錶達率分彆為11.9%±3.4%、10.6%±2.9%和9.5%±3.6%,肝包蟲活性組與正常對照組PD-L1的暘性錶達率比較,差異有統計學意義(t=2.470,P<0.05).肝包蟲活性組、肝包蟲無活性組和正常對照組血清IFN-γ錶達分彆為(141±38) μg/L、(124±32) μg/L和(105±42) μg/L,肝包蟲活性組與正常對照組IFN-γ錶達水平比較,差異有統計學意義(t=3.280,P<0.05).流式細胞儀和免疫組織化學檢測PBMC的PD-L1暘性錶達率與血清IFN-γ錶達水平均呈明顯正相關(r=0.59,0.61,P<0.05).結論 PD-L1可能在IFN-γ的作用下髮揮著促進肝包蟲免疫逃避的重要作用.
목적 탐토간낭형포충병환자외주혈단개핵세포(PBMC)표면정서성사망수체배체1(PD-L1)적표체급기여혈청1FN-γ수평지간적관계.방법 회고성분석2010년6월지2011년2월신강의과대학제일부속의원수치적63례간낭형포충병환자적림상자료.근거세계위생조직포충병전가조포충병표준화분형원칙장환자분위간포충활성조(38례)화간포충무활성조(25례),20례간혈관류환자급건강지원자작위정상대조조.채용류식세포의급면역조직화학법검측PD-L1양성표체솔,ELISA법검측혈청IFN-γ적표체수평.조간비교채용성조설계자료£검험화단인소방차분석,진일보량량비교채용LSD검험,총체분포채용x2검험,PD-LI양성표체솔화IFN-γ적표체수평적상관성분석채용Pearson검험.결과 류식세포의검측간포충활성조、간포충무활성조화정상대조조PBMC표면PD-L1적양성표체솔분별위12.1%사3.8%、10.9%사2.5%화9.1%±2.5%,간포충활성조여정상대조조PD-L1적양성표체솔비교,차이유통계학의의(t =3.327,P<0.05).면역조직화학검측간포충활성조、간포충무활성조화정상대조조PBMC도편중PD-L1적양성표체솔분별위11.9%±3.4%、10.6%±2.9%화9.5%±3.6%,간포충활성조여정상대조조PD-L1적양성표체솔비교,차이유통계학의의(t=2.470,P<0.05).간포충활성조、간포충무활성조화정상대조조혈청IFN-γ표체분별위(141±38) μg/L、(124±32) μg/L화(105±42) μg/L,간포충활성조여정상대조조IFN-γ표체수평비교,차이유통계학의의(t=3.280,P<0.05).류식세포의화면역조직화학검측PBMC적PD-L1양성표체솔여혈청IFN-γ표체수평균정명현정상관(r=0.59,0.61,P<0.05).결론 PD-L1가능재IFN-γ적작용하발휘착촉진간포충면역도피적중요작용.
Objective To investigate the expression of programmed death receptor ligand 1 ( PD-L1 ) of peripheral blood mononuclear cells (PBMCs) in patients with hepatic cystic echincccccosis (HCE) and its relation with interferon-γ.Methods The clinical data of 63 patients with HCE who were admitted to the First Affiliated Hospital of Xinjiang Medical University from June 2010 to February 2011 were retrospectively analyzed.All patients were divided into HCE active group (38 patients) and HCE non-active group (25 patients) according to the system established by the World Health Organization's Informal Working Group on Echinocoecosis.Twenty patients with hepatic hemangioma or healthy individuals were recruited in normal control group.The positive rate of PD-L1 expression was detected by flow cytometry and immunocytochemistry.The expression of interferon-γ was detected by enzyme-linked immtmosorbent assay (ELISA).All data were analyzed by the t test,one-way analysis of variance,LSD test and chi-square test.The relationship between the expression of interferon-γ and positive rate of PD-L1 expression was analyzed by the Pearson test.Results The results of flow cytometry showed that the positive rates of PD-L1 expression in the HCE active group,HCE non-active group and normal control group were 12.1%±3.8%,10.9% ± 2.5% and 9.1% ±2.5%,respectively.There was a significant difference in the positive rate of PD-L1 expression between the HCE active group and normal control group (t =3.327,P < 0.05 ).The results of immunohistochemistry showed that the positive rates of PD-LI expression in the HCE active group,HCE non-active group and normal control group were 11.9% ± 3.4%,i0.6% ± 2.9% and 9.5% ± 3.6%,respectively.There was a significant difference in the positive rate of PD-L1 expression between the HCE active group and normal control group (t =2.470,P < 0.05 ).The expressions of intefferon-γ in the HCE active group,HCE non-active group and normal control group were ( 141 ± 38 ) μμg/L,( 124 ± 32 ) μg/L and ( 105 ± 42 ) μg/L.There was asignificant difference in the expression of interferon-γ between the HCE active group and normal control group ( t =3.280,P < 0.05).The results of flow cytometry and immunohistochemistry revealed that the positive rate of PD-L1 expression was positively correlated with the expression of interferon-γ( r =0.59,0.61,P < 0.05 ).Conclusion With the help of interferon-γ,PD-L1 may play an important role in promoting the immune.evasion of echinococcus.