中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
11期
1037-1041
,共5页
王辉%谭艳%朱秀云%廖明凤%张洁云%刘艳%刘水腾%张路坤%周映%刘映霞%周伯平%陈心春%卢洪州
王輝%譚豔%硃秀雲%廖明鳳%張潔雲%劉豔%劉水騰%張路坤%週映%劉映霞%週伯平%陳心春%盧洪州
왕휘%담염%주수운%료명봉%장길운%류염%류수등%장로곤%주영%류영하%주백평%진심춘%로홍주
人类免疫缺陷病毒%结核潜伏感染%IFN-γ释放实验%酶联免疫斑点技术%结核菌素皮肤试验
人類免疫缺陷病毒%結覈潛伏感染%IFN-γ釋放實驗%酶聯免疫斑點技術%結覈菌素皮膚試驗
인류면역결함병독%결핵잠복감염%IFN-γ석방실험%매련면역반점기술%결핵균소피부시험
Human immunodeficiency virus%Latent tuberculosis infection%IFN-γ release assay%Enzyme-linked immunospot assay%Tuberculin skin test
目的 探讨结核分枝杆菌抗原特异性IFN-γ酶联免疫斑点(ELISPOT)检测技术在HIV感染者中用于诊断结核潜伏感染的应用价值.方法 运用自行研发的结核分枝杆菌抗原特异性IFN-γ ELISPOT检测方法对110例确诊的HIV感染者及205例HIV阴性健康志愿者的外周血结核特异性IFN-γ释放水平进行检测,并同时进行结核菌素皮肤试验(研).结果 ELISPOT在HIV感染和HIV/AIDS正在接受抗病毒治疗人群中的阳性率分别为24.5%和29.8%,明显高于HIV阴性健康人群7.3%(P＜0.001);对多种不同部位多肽抗原刺激以P8.10的敏感性最高,但与其他抗原的差异无统计学意义(P＞0.05);不同CD4细胞计数水平对ELISPOT检测结果尤影响(P＞0.05);PPD试验在HIV感染人群中的阳性率远远低于ELISPOT检测方法,其差异有统计学意义(P＜0.0001).结论 结核分枝杆菌抗原特异性IFN-γ ELISPOT检测技术在艾滋病合并结核潜伏感染的早期诊断中有很大的应用价值,推荐用于艾滋病合并结核潜伏感染的辅助诊断.
目的 探討結覈分枝桿菌抗原特異性IFN-γ酶聯免疫斑點(ELISPOT)檢測技術在HIV感染者中用于診斷結覈潛伏感染的應用價值.方法 運用自行研髮的結覈分枝桿菌抗原特異性IFN-γ ELISPOT檢測方法對110例確診的HIV感染者及205例HIV陰性健康誌願者的外週血結覈特異性IFN-γ釋放水平進行檢測,併同時進行結覈菌素皮膚試驗(研).結果 ELISPOT在HIV感染和HIV/AIDS正在接受抗病毒治療人群中的暘性率分彆為24.5%和29.8%,明顯高于HIV陰性健康人群7.3%(P＜0.001);對多種不同部位多肽抗原刺激以P8.10的敏感性最高,但與其他抗原的差異無統計學意義(P＞0.05);不同CD4細胞計數水平對ELISPOT檢測結果尤影響(P＞0.05);PPD試驗在HIV感染人群中的暘性率遠遠低于ELISPOT檢測方法,其差異有統計學意義(P＜0.0001).結論 結覈分枝桿菌抗原特異性IFN-γ ELISPOT檢測技術在艾滋病閤併結覈潛伏感染的早期診斷中有很大的應用價值,推薦用于艾滋病閤併結覈潛伏感染的輔助診斷.
목적 탐토결핵분지간균항원특이성IFN-γ매련면역반점(ELISPOT)검측기술재HIV감염자중용우진단결핵잠복감염적응용개치.방법 운용자행연발적결핵분지간균항원특이성IFN-γ ELISPOT검측방법대110례학진적HIV감염자급205례HIV음성건강지원자적외주혈결핵특이성IFN-γ석방수평진행검측,병동시진행결핵균소피부시험(연).결과 ELISPOT재HIV감염화HIV/AIDS정재접수항병독치료인군중적양성솔분별위24.5%화29.8%,명현고우HIV음성건강인군7.3%(P＜0.001);대다충불동부위다태항원자격이P8.10적민감성최고,단여기타항원적차이무통계학의의(P＞0.05);불동CD4세포계수수평대ELISPOT검측결과우영향(P＞0.05);PPD시험재HIV감염인군중적양성솔원원저우ELISPOT검측방법,기차이유통계학의의(P＜0.0001).결론 결핵분지간균항원특이성IFN-γ ELISPOT검측기술재애자병합병결핵잠복감염적조기진단중유흔대적응용개치,추천용우애자병합병결핵잠복감염적보조진단.
Objective To assess the validity of a newly developed in-house ELISPOT IFN-γ release assay (IGRA) for the detection of latent tuberculosis infection among HIV infected individuals. Methods In-house ELISPOT assay were performed, together with a tuberculin skin test in 205 health controls and 110 HIV infected individuals , who had no signs of active tuberculosis at time of enrolment . Results Using the ELISPOT assay, positivity rates for the 205 health controls, 110 HIV infected individuals and 47 AIDS patients on highly active antiretrovial therapy (HAART) were 7. 3% , 24.5% , 29. 8% , respectively. These results indicated that the positive rates obtained from HIV infected individuals (include patient on HAART) was significantly higher than health controls( P ＜ 0.001). We found no significant correlation between the CD4 cell count and positivity of ELISPOT assay (P ＞0.05 ). The proportion of subjects with a positive response to ELISPOT assay were higher than the proportion of tuberculin skin test(TST) responders(P＜0.0001) in HIV infected individuals. Conclusion Our study indicates that IGRA using M. tuberculosis specific antigens are likely to retain their validity for the diagnosis of LTBI among HIV positive individuals.
