中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
4期
288-291
,共4页
毕淑娜%戴淑真%姚勤%车艳辞%王宁%杨堃
畢淑娜%戴淑真%姚勤%車豔辭%王寧%楊堃
필숙나%대숙진%요근%차염사%왕저%양곤
间皮素%卵巢肿瘤%黏附%逆转录聚合酶链反应%免疫组织化学
間皮素%卵巢腫瘤%黏附%逆轉錄聚閤酶鏈反應%免疫組織化學
간피소%란소종류%점부%역전록취합매련반응%면역조직화학
Mesothelin%Ovarian neoplasms%Adhesion%RT-PCR%Immunohistochemistry
目的 研究间皮素(MESO)在卵巢癌中的表达及意义.方法 用逆转录聚合酶链反应(RT-PCR)技术和免疫组化方法分别检测卵巢肿瘤和正常卵巢组织中MESO mRNA及其蛋白水平.结果MESO mRNA和蛋白在上皮性卵巢癌(1.4005 ±0.4646,2.7857±2.2712)和交界性卵巢肿瘤(1.0650 ±0.3100,2.9167 ±2.391)中的表达水平高于良性卵巢肿瘤(0.6463±0.2419,1.2500 ±1.6125)和正常卵巢组织(0.6439 ±0.2729,0.9167 ±1.2401),差异有统计学意义(P＜0.05);MESOmRNA和蛋白在浆液性卵巢癌(1.5255 ±0.4151,3.3036 ±2.6141)和子宫内膜样癌(1.5250 ±0.5419,3.0000 ±2.3094)中的表达水平高于黏液样癌(1.0675±0.3149,1.0556 ±1.9242),差异有统计学意义(P＜0.05);临床Ⅲ、Ⅳ期MESO表达水平(1.5100 ±0.4142,3.6087 ±3.3959)高于Ⅰ、Ⅱ期(1.1190 ±0.4909,1.7895 ±2.6320),差异有统计学意义(P＜0.05).MESO表达水平与病理分级有关(P＜0.05),而与患者年龄和血清CA125水平无关(P＞0.05).结论 MESO mRNA及蛋白在卵巢癌和交界性肿瘤组表达增高,MESO可能参与卵巢癌的黏附转移.
目的 研究間皮素(MESO)在卵巢癌中的錶達及意義.方法 用逆轉錄聚閤酶鏈反應(RT-PCR)技術和免疫組化方法分彆檢測卵巢腫瘤和正常卵巢組織中MESO mRNA及其蛋白水平.結果MESO mRNA和蛋白在上皮性卵巢癌(1.4005 ±0.4646,2.7857±2.2712)和交界性卵巢腫瘤(1.0650 ±0.3100,2.9167 ±2.391)中的錶達水平高于良性卵巢腫瘤(0.6463±0.2419,1.2500 ±1.6125)和正常卵巢組織(0.6439 ±0.2729,0.9167 ±1.2401),差異有統計學意義(P＜0.05);MESOmRNA和蛋白在漿液性卵巢癌(1.5255 ±0.4151,3.3036 ±2.6141)和子宮內膜樣癌(1.5250 ±0.5419,3.0000 ±2.3094)中的錶達水平高于黏液樣癌(1.0675±0.3149,1.0556 ±1.9242),差異有統計學意義(P＜0.05);臨床Ⅲ、Ⅳ期MESO錶達水平(1.5100 ±0.4142,3.6087 ±3.3959)高于Ⅰ、Ⅱ期(1.1190 ±0.4909,1.7895 ±2.6320),差異有統計學意義(P＜0.05).MESO錶達水平與病理分級有關(P＜0.05),而與患者年齡和血清CA125水平無關(P＞0.05).結論 MESO mRNA及蛋白在卵巢癌和交界性腫瘤組錶達增高,MESO可能參與卵巢癌的黏附轉移.
목적 연구간피소(MESO)재란소암중적표체급의의.방법 용역전록취합매련반응(RT-PCR)기술화면역조화방법분별검측란소종류화정상란소조직중MESO mRNA급기단백수평.결과MESO mRNA화단백재상피성란소암(1.4005 ±0.4646,2.7857±2.2712)화교계성란소종류(1.0650 ±0.3100,2.9167 ±2.391)중적표체수평고우량성란소종류(0.6463±0.2419,1.2500 ±1.6125)화정상란소조직(0.6439 ±0.2729,0.9167 ±1.2401),차이유통계학의의(P＜0.05);MESOmRNA화단백재장액성란소암(1.5255 ±0.4151,3.3036 ±2.6141)화자궁내막양암(1.5250 ±0.5419,3.0000 ±2.3094)중적표체수평고우점액양암(1.0675±0.3149,1.0556 ±1.9242),차이유통계학의의(P＜0.05);림상Ⅲ、Ⅳ기MESO표체수평(1.5100 ±0.4142,3.6087 ±3.3959)고우Ⅰ、Ⅱ기(1.1190 ±0.4909,1.7895 ±2.6320),차이유통계학의의(P＜0.05).MESO표체수평여병리분급유관(P＜0.05),이여환자년령화혈청CA125수평무관(P＞0.05).결론 MESO mRNA급단백재란소암화교계성종류조표체증고,MESO가능삼여란소암적점부전이.
Objective To investigate the expression of mesothelin(MESO)mRNA and protein and its significance in ovarian carcinomas.Methods Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR)and immunohistochemistry were used to detect the expression level of MESO mRNA and protein,respectively,in 124 samples of ovarian tumor and normal tissues,including 84 epithelial ovarian carcinomas.12 borderline ovarian tumors.16 benign ovarian tumors and 12 normal ovarian tissues.Results The expression of MESO mRNA and protein in epithelial ovarian carcinomas(1.4005 ±0.4646.2.7857 ±2.2712)and bordertine ovarian tumors(1.0650 ±0.3100,2.9167 ±2.391)were significantly higher than that in benign ovarian tumors(0.6463±0.2419.1.2500 ±1.6125)and normal ovarian tissues(0.6439 ±0.2729,0.9167 ±1.2401)(P＜0.05),and also significantly higher in serous cystadenocarcinoma(1.5255 ±0.4151.3.3036 ±2.6141)and endometrioid carcinoma(1.5250 ±0.5419.3.0000 ±2.3094)than that in mucinous cystadenocarcinoma(1.0675 ±0.3149.1.0556 ±1.9242)(P＜0.05).The expression of MESO mRNA and protein in stages Ⅲ and Ⅳ carcinomas(1.5100 ±0.4142,3.6087 ±3.3959)was significantly higher than that in stages Ⅰ andⅡcarcinomas(1.1190 ±0.4909,1.7895 ±2.6320;P＜0.05),and also significantly higher in grade 3 carcinomas than that in grade 1 and 2 ones(P＜0.05),but was not correlate with age or serum CAl25 of the patients(P＞0.05).Conclusion The results of this study demonstrated that the expression of MESO mRNA and protein is increased in ovarian carcinomas and borderline ovarian tumors.and MESO may play a role in the adhesion and dissemination of ovarian carcinomas.
