CAJ | 학술논문

目的将新型可注射水凝胶植入心肌梗死区,观察其对心室重构和心功能的影响,探讨新型生物材料治疗心肌梗死的可行性和理论基础.方法将16只雄性日本大耳白兔通过结扎左冠状动脉建立心肌梗死模型,并随机分为水凝胶注射组和对照组,1周后将200μI可注射水凝胶聚乙二醇-己内酯/ α-环糊精(MPM/ α-CO)多点注射到心肌梗死区,对照组注射200μI磷酸盐缓冲溶液(PBS),28 d后进行超声心动图检查、组织学及免疫组织化学检测.结果水凝胶注射组左室射血分数(56.1%4±8.4%)明显高于对照组(37.3%4±6.4%,P<0.05),组织学染色显示水凝胶注射4周后已降解吸收,无明显残留,水凝胶注射组梗死面积(22.48%4±3.30%)小于对照组(29.02%4±3.02%,P<0.05),水凝胶注射组梗死壁厚度[(3.08±0.32)mm]大于对照组[(2.18 4±0.46)mm,P<0.05].但水凝胶注射组新生血管数[(100.8 4±2.4)/ mm2]与对照组[(98.5±2.9)/ mm2]比较差异无统计学意义(P>0.05).结论 MPM/ α-CD水凝胶注射到心肌梗死部位可防止梗死扩展和心室扩大,延缓心室重构,改善心功能,可注射水凝胶植入可能是治疗心肌梗死的全新有效策略.
목적 장신형가주사수응효식입심기경사구,관찰기대심실중구화심공능적영향,탐토신형생물재료치료심기경사적가행성화이론기출.방법 장16지웅성일본대이백토통과결찰좌관상동맥건립심기경사모형,병수궤분위수응효주사조화대조조,1주후장200μI가주사수응효취을이순-기내지/ α-배호정(MPM/ α-CO)다점주사도심기경사구,대조조주사200μI린산염완충용액(PBS),28 d후진행초성심동도검사、조직학급면역조직화학검측.결과 수응효주사조좌실사혈분수(56.1%4±8.4%)명현고우대조조(37.3%4±6.4%,P<0.05),조직학염색현시수응효주사4주후이강해흡수,무명현잔류,수응효주사조경사면적(22.48%4±3.30%)소우대조조(29.02%4±3.02%,P<0.05),수응효주사조경사벽후도[(3.08±0.32)mm]대우대조조[(2.18 4±0.46)mm,P<0.05].단수응효주사조신생혈관수[(100.8 4±2.4)/ mm2]여대조조[(98.5±2.9)/ mm2]비교차이무통계학의의(P>0.05).결론 MPM/ α-CD수응효주사도심기경사부위가방지경사확전화심실확대,연완심실중구,개선심공능,가주사수응효식입가능시치료심기경사적전신유효책략.
Objective To observe whether injectable hydrogel implantation could prevent adverse cardiac remodeling and preserve cardiac function in rabbits with myocardial infarction (MI). Methods A novel injectable hydrogel, the copolymer MPM/α-CD, which self-assembled between α-cyclodextrin and methoxy polyethylene glycol-poly (caprolactone)-(dodecanedioic acid)-poly (caprolactone)-methoxypolyethylene glycol triblock polymer, was synthesized by chemical crosslinking and characterized by biocompatibility and biodegradability. Experimental MI was induced in male rabbits by coronary artery ligation. The MI rabbits were randomly divided into hydrogel group (200 μl MPM/α-CD were injected into the infarcted myocardium 7 days after MI) and control group (equal volume phosphate buffered saline myocardial injection, n=8 each). Four weeks after MPM/α-CD implantation, echocardiography,histochemistry and immunohistochemistry were performed. Results Left ventricle ejection fraction was significantly improved in the hydrogel-treated group (56.1% ± 8.4%) than that in the control group (37.3% ±6.4%, P <0.05). Histological analysis indicated that hydrogel degraded 4 weeks after hydrogel injection, and prevented scar expansion and wall thinning [(3.08 ± 0.32) mm vs. (2.18 ± 0.46) mm in control group, P <0.05]. Neovasculature formation was similar between the hydrogel group [(100.8 ± 2.4)/mm2] and the control [(98.5 ± 2.9)/mm2, P>0.05]. onclusion MPM/α-CD could serve as an excellent injectable biomaterial for improves cardiac function and attenuating scar expansion and left ventricular dilation in MI rabbits.