CAJ | 학술논문

目的探讨精子染色质结构异常对体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)的影响.方法对IVF-ET治疗的不育夫妇136对,分别检测精子DNA碎片化和染色质包装缺陷,并分析精子染色质结构参数与受精及临床妊娠之间的关系.结果精子DNA碎片化阳性率和染色质包装缺陷阳性率均与受精率呈负相关(r值分别为-0.198,P＜0.05,和-0.389,P＜0.01);在未获得临床妊娠的患者,精子DNA碎片化阳性率和染色质包装缺陷阳性率均高于获得临床妊娠的夫妇(分别为10.74%vs.5.40%,P＜0.01和23.58%vs.11.83%,P＜0.01).结论 IVF-ET治疗失败与精子染色质结构异常有关,精子染色质结构检测有助于评估IVF-ET失败的风险,以制订最佳治疗方案.
목적 탐토정자염색질결구이상대체외수정-배태이식(in vitro fertilization and embryo transfer,IVF-ET)적영향.방법 대IVF-ET치료적불육부부136대,분별검측정자DNA쇄편화화염색질포장결함,병분석정자염색질결구삼수여수정급림상임신지간적관계.결과 정자DNA쇄편화양성솔화염색질포장결함양성솔균여수정솔정부상관(r치분별위-0.198,P＜0.05,화-0.389,P＜0.01);재미획득림상임신적환자,정자DNA쇄편화양성솔화염색질포장결함양성솔균고우획득림상임신적부부(분별위10.74%vs.5.40%,P＜0.01화23.58%vs.11.83%,P＜0.01).결론 IVF-ET치료실패여정자염색질결구이상유관,정자염색질결구검측유조우평고IVF-ET실패적풍험,이제정최가치료방안.
Objective To explore the effects of sperm chromatin structure abnormalities on the outcome of in vitro fertilization and embryo transfer (IVF-ET). Methods Sperm DNA fragmentation and chromatin packaging defects were assessed in 136 couples undergoing IVF-ET because of infertility. The relationship between sperm DNA fragmentation, chromatin packaging defects and fertilization rate and clinical pregnancy was evaluated. Results Both sperm DNA fragmentation and chromatin packaging defect had a negative correlation with fertilization rate (r= -0. 198, P＜0. 05, and r= -0. 389, P＜0. 01,respectively). Both parameters were higher in couples who failed to achieve pregnancy than those who achieved clinical pregnancy (10. 74% vs. 5. 40%, P＜0. 01 and 23. 58% vs. 11. 83%, P＜0. 01,respectively). Conclusion Abnormality of sperm chromatin structure is one of the reasons for IVF-ET failure. Examination of sperm chromatin structure is helpful in predicting he risk of IVF-ET failure and optimizing treatment of infertility.