CAJ | 학술논문

目的应用反义基因转染技术探讨肾癌相关新基因GYLZ-RCC18与肾癌细胞死亡（包括坏死及凋亡）之间的关系，并探讨肾癌基因疗法的新途径。方法用脂质体包裹的新基因GYLZ-RCC18第一编码区起始处的20个碱基的反义和正义寡核苷酸导入肾癌细胞系GRC-1，应用流式细胞技术和伊红排斥实验连续检测GYLZ-RCC18反义寡核苷酸对肾癌细胞凋亡、坏死的影响。结果导入GYLZ-RCC18反义寡核苷酸后，可大量杀伤GRC-1细胞并明显促进GRC-1细胞坏死，于第4天达到杀伤高峰约60%；同时可连续8 d诱导凋亡峰，最高于第2天达到约22.5%，两种作用曲线形状和峰值不完全一致。结论 GYLZ-RCC18是肾癌相关的重要的新的癌基因，GYLZ-RCC18的表达可通过两种不同的机制即抗癌细胞坏死机制和抗凋亡机制发挥对肾癌细胞的保护作用，GYLZ-RCC18可能对肾癌细胞生存和耐药至关重要。通过导入反义基因的方法阻断肾癌细胞中GYLZ-RCC18的表达，可有效地杀伤肾癌细胞。对此新基因的研究将有助于为肾癌的基础研究和临床治疗提供新的思路和途径。
목적응용반의기인전염기술탐토신암상관신기인GYLZ-RCC18여신암세포사망（포괄배사급조망）지간적관계，병탐토신암기인요법적신도경。방법용지질체포과적신기인GYLZ-RCC18제일편마구기시처적20개감기적반의화정의과핵감산도입신암세포계GRC-1，응용류식세포기술화이홍배척실험련속검측GYLZ-RCC18반의과핵감산대신암세포조망、배사적영향。결과도입GYLZ-RCC18반의과핵감산후，가대량살상GRC-1세포병명현촉진GRC-1세포배사，우제4천체도살상고봉약60%；동시가련속8 d유도조망봉，최고우제2천체도약22.5%，량충작용곡선형상화봉치불완전일치。결론 GYLZ-RCC18시신암상관적중요적신적암기인，GYLZ-RCC18적표체가통과량충불동적궤제즉항암세포배사궤제화항조망궤제발휘대신암세포적보호작용，GYLZ-RCC18가능대신암세포생존화내약지관중요。통과도입반의기인적방법조단신암세포중GYLZ-RCC18적표체，가유효지살상신암세포。대차신기인적연구장유조우위신암적기출연구화림상치료제공신적사로화도경。
al cell carcinoma cell line GRC-1 and study its role in the development of RCC.　Methods　20 bp GYLZ-RCC18 antisense oligonuclotide packed with liposome was transfected into GRC-1 cell and the changes in apoptosis and mortality of GRC-1 were observed by using flow cytometry and staining.　Results　After transfection of GYLZ-RCC18 antisense oligonuclotide, the mortality of GRC-1 increased evidently. The antisense oligonuclotide induced the apoptosis of GRC-1.　Clonclusions　GYLZ-RCC18 is one of RCC closely related novel gene. It may exert an antidead and antiapoptosis effect on RCC. It is also related to the multidrug resistance of RCC. Transfection of antisense oligonuclotide could inhibite the development of RCC. The study of this gene provides a new clue for the study and gene therapy of RCC.