CAJ | 학술논문

目的探讨HIV-1感染疾病缓慢进展者CD8+T淋巴细胞非细胞毒性抗病毒应答功能(CNAR)的变化.方法应用密度梯度离心法、免疫磁珠法纯化健康人CD4+T淋巴细胞和HIV感染者CD8+T淋巴细胞,用HIV毒株SF-33感染健康人CD4+T淋巴细胞,并加入不同疾病进程HIV感染者CD8+T淋巴细胞共培养,收集培养上清,应用ELISA方法测定上清中HIV-1 p24含量.结果我们研究发现缓慢进展组(slow progressors,SP)、HIV典型进展组(typical progressors,TP)、健康对照组及AIDS组中CNAR功能依次下降(89%>77%>73%>61%),各组间的下降差异均有统计学意义(P<0.05);在HIV感染者中,CNAR功能与CD4+T细胞绝对计数呈显著正相关;与病毒载量无显著相关性.结论 CNAR功能对HIV感染疾病不进展可能具有保护作用.
목적 탐토HIV-1감염질병완만진전자CD8+T림파세포비세포독성항병독응답공능(CNAR)적변화.방법 응용밀도제도리심법、면역자주법순화건강인CD4+T림파세포화HIV감염자CD8+T림파세포,용HIV독주SF-33감염건강인CD4+T림파세포,병가입불동질병진정HIV감염자CD8+T림파세포공배양,수집배양상청,응용ELISA방법측정상청중HIV-1 p24함량.결과 아문연구발현완만진전조(slow progressors,SP)、HIV전형진전조(typical progressors,TP)、건강대조조급AIDS조중CNAR공능의차하강(89%>77%>73%>61%),각조간적하강차이균유통계학의의(P<0.05);재HIV감염자중,CNAR공능여CD4+T세포절대계수정현저정상관;여병독재량무현저상관성.결론 CNAR공능대HIV감염질병불진전가능구유보호작용.
Objective To investigate the change of CD8+T lymphocyte non-cytotoxic antivirus response(CNAR)in slow progressors infected by HIV.Methods Applying with density gradient and immunomagnetic beads methods to purify the CD4+T lymphocyte from the healthy person and CD8+T lymphocyte from HIV-infected individuals.The CD4+T cell was infected by HIV(SF-33)virus and cocuhured with CD8+T cell.The culture supernatant was collected and the p24 value was detected by ELISA method.Results Our study showed that the CNAR function decreased by turns of slow progressors(SP),typical progressors(TP),health control group and AIDS group.There was significant difference between groups(P<0.01).We found a significant positive correlation between the CIM+T cell ture count and the CNAR function.The virus load didn't statistically correlate with the CNAR function.Conclusion The CNAR function possibly protected the HIV-infected individuals from progression.