CAJ | 학술논문

目的阐明人呼吸道合胞病毒(human respiratory syncytial virus,HRSV)A和B亚型病毒分离株的核蛋白(nucleoprotein,N)编码基因特征.方法采用逆转录-聚合酶链反应(revelrse transciptionpolymerase chain reaction,RT-PCR)对北京2004年分离的HRSV代表株(2株A亚型和2株B亚型)进行N基因全长序列扩增、测序,并和GenBank下载的所有HRSV病毒的N基因全长序列进行对比和分析.结果 2株HRSV A亚型分离株与A亚型Long株(标准株)的N蛋白的核苷酸和氨基酸差异分别为36～40个(3.1%～3.4%)和4个(1.0%);2株HRSV B亚型分离株与B亚型CH18537株(标准株)的N蛋白之间的核苷酸和氨基酸差异分别为17(1.4%)和1(0.3%)个.4株HRSV代表株和从GenBank下载的HRSV的N蛋白之间的核苷酸和氨基酸差异分别为3～172个(0.25%～14.63%)和0-18个(0～4.6%).结论 N基因在HRSV基因组中是较为保守的基因,A或B亚型的型内差异相对较小;但A和B亚型之间N基因序列有较大变异,变异平均分配于整个N基因中;本研究为HRSV基因快速诊断试剂的研制提供了基因信息学数据.
목적 천명인호흡도합포병독(human respiratory syncytial virus,HRSV)A화B아형병독분리주적핵단백(nucleoprotein,N)편마기인특정.방법 채용역전록-취합매련반응(revelrse transciptionpolymerase chain reaction,RT-PCR)대북경2004년분리적HRSV대표주(2주A아형화2주B아형)진행N기인전장서렬확증、측서,병화GenBank하재적소유HRSV병독적N기인전장서렬진행대비화분석.결과 2주HRSV A아형분리주여A아형Long주(표준주)적N단백적핵감산화안기산차이분별위36～40개(3.1%～3.4%)화4개(1.0%);2주HRSV B아형분리주여B아형CH18537주(표준주)적N단백지간적핵감산화안기산차이분별위17(1.4%)화1(0.3%)개.4주HRSV대표주화종GenBank하재적HRSV적N단백지간적핵감산화안기산차이분별위3～172개(0.25%～14.63%)화0-18개(0～4.6%).결론 N기인재HRSV기인조중시교위보수적기인,A혹B아형적형내차이상대교소;단A화B아형지간N기인서렬유교대변이,변이평균분배우정개N기인중;본연구위HRSV기인쾌속진단시제적연제제공료기인신식학수거.
Objective To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.Methods Reverse transciption polymerage chain reaction(RT- PCR)was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004.The RT-PCR products were sequenced for N protein coding region.The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.Results The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36.40(3.1%-3.4%)and 4(1.0%).The difierences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17(1.4%)and 1(0.3%).The differences in number of nucleotide and deduced amino acid were 3-172(0.25%-14.63%)and 0-18(0-4.6%)respectively between 4 Beijing 2004 isolates and GenBank sequences.Conclusion N gene is the hishly conservative gene in the HRSV genome.The variation between A and B subgroups were widely distributed in the entire gene of N protein,while the variation within the A or B subgroups HRSV wag considerably lower.