CAJ | 학술논문

目的观察强制性运动训练对帕金森病(PD)模型大鼠酪氨酸羟化酶(TH)及胶质细胞源性神经营养因子(GDNF)表达的影响.方法共选取50只SD大鼠,采用6-羟基多巴胺(6-OHDA)单侧两点注射法制备PD动物模型,l周后将制模成功的42只大鼠随机分为运动组及对照组.运动组通过石膏固定健侧前肢,从而强制大鼠使用患侧肢体；对照组制模后未给予特殊处理.待2周训练结束后,每组大鼠腹腔注射阿朴吗啡(APO)进行行为学测试,记录30 min内转圈数量；然后处死大鼠取脑纹状体制成标本,采用高效液相-电化学法测定各组大鼠纹状体内多巴胺(DA)及其代谢物3,4-二羟基苯乙酸(DOPAC)含量；采用免疫组化技术检测各组大鼠纹状体TH及GDNF表达情况,采用逆转录-聚合酶链反应(RT-PCR)测定TH及GDNF mRNA 表达.结果与对照组比较,运动组在APO诱导下30 min内转圈数量显著减少(P＜0.05)；运动组纹状体匀浆中DA及其代谢物DOPAC含量显著增加(P＜0.05)；运动组纹状体TH、GDNF蛋白及mRNA表达均显著强于对照组(P＜0.05).结论强制性运动训练可改善PD大鼠行为学功能,其机制可能与促进大脑纹状体TH及GDNF表达、提高DA及其代谢产物DOPAC含量有关.
목적 관찰강제성운동훈련대파금삼병(PD)모형대서락안산간화매(TH)급효질세포원성신경영양인자(GDNF)표체적영향.방법 공선취50지SD대서,채용6-간기다파알(6-OHDA)단측량점주사법제비PD동물모형,l주후장제모성공적42지대서수궤분위운동조급대조조.운동조통과석고고정건측전지,종이강제대서사용환측지체；대조조제모후미급여특수처리.대2주훈련결속후,매조대서복강주사아박마배(APO)진행행위학측시,기록30 min내전권수량；연후처사대서취뇌문상체제성표본,채용고효액상-전화학법측정각조대서문상체내다파알(DA)급기대사물3,4-이간기분을산(DOPAC)함량；채용면역조화기술검측각조대서문상체TH급GDNF표체정황,채용역전록-취합매련반응(RT-PCR)측정TH급GDNF mRNA 표체.결과 여대조조비교,운동조재APO유도하30 min내전권수량현저감소(P＜0.05)；운동조문상체균장중DA급기대사물DOPAC함량현저증가(P＜0.05)；운동조문상체TH、GDNF단백급mRNA표체균현저강우대조조(P＜0.05).결론 강제성운동훈련가개선PD대서행위학공능,기궤제가능여촉진대뇌문상체TH급GDNF표체、제고DA급기대사산물DOPAC함량유관.
Objective To explore the effects of constraint-induced movement therapy (CIMT) on the expression of tyrosine hydroxylase (TH) and glial cell derived neurotrophic factor (GDNF) in Parkinson's disease (PD) model rats. Methods PD models were established by microinjection of 6-hydroxydopamine (6-OHDA) solution into substantia nigra of rats' right cerebral hemisphere.Forty-two model rats were divided randomly into an exercise group and a control group 1 week after microinjection.The exercise group rats were forced to use their impaired limbs by placing their nonimpaired fore-limbs in casts.The control group rats were housed in the same environment without any special treatment.Two weeks after 6-OHDA infusion and exercise training,the behavioral changes of rats were examined after intraperitoneal injection apomorphine ( APO).The content of dopamine (DA) and dihydroxyphenylacetic acid (DOPAC) was measured by high performance liquid chromatography with electrochemistry ( HPLAEC) ; the expressions of TH and GDNF in striatum were detected by immunohistochemical methods and TH,GDNF mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results After 2 weeks of training,the rotating laps of the rats in exercise group within 30 min after APO induction,reduced to a significantly greater extent when compared to the control group (P ＜ 0.05).The content of DA and it's metabolites DOPAC in striatum homogenate was significantly higher in exercise group than that in the control group ( P ＜ 0.05 ),and the expression levels,of TH and GDNF protein/ mRNA were also significantly higher in the exercise group than those in control group ( P ＜ 0.05 ). Conclusions CIMT can improve the behavioral performance of PD rats,probably through promoting the expressions of TH and GDNF protein/mRNA in striatum,and increasing DA and it's metabolites DOPAC level.