中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
3期
223-225
,共3页
李泽良%曲怀刚%马春媚%何宏文
李澤良%麯懷剛%馬春媚%何宏文
리택량%곡부강%마춘미%하굉문
阿尔茨海默病%当归芍药散%快速老化小鼠%学习记忆
阿爾茨海默病%噹歸芍藥散%快速老化小鼠%學習記憶
아이자해묵병%당귀작약산%쾌속노화소서%학습기억
Alzheimer's disease%Danggui Shaoyao San%SAM-P8 mice%Learning and memory
目的 观察当归芍药散(DSS)对快速老化小鼠空间学习记忆能力的影响,为当归芍药散治疗阿尔采默病提供实验依据.方法 选用雌性SAM-P8鼠 36只,分成4组:预防组(3月龄)、预防对照组(3月龄)、治疗组(6月龄)、治疗对照组(6月龄),每组8只.治疗组以DSS(浓度为0.5g/ml)灌胃1个月,预防组自由饮用DSS(0.02%,V/V)4个月,对照组给予冷开水,给药结束后行Morris水迷宫测试.结果 Morris水迷宫测试预防组、预防对照组、治疗组、治疗对照组的平均逃避潜伏期分别是(41.29±20.33)s、(62.17±20.36)s、(39.67±19.60)s、(63.00±22.16)s;穿越平台次数分别是(6.13±2.21)次、(2.04±1.42)次、(5.41±2.13)次、(3.12±1.20)次;平台象限游泳距离百分比分别是(51.14±12.38)%、(30.75±14.03)%、(47.11±9.29)%、(33.17±10.45)%.上述指标在预防组、治疗组与其相应对照组比较差异有显著性( P <0.05).结论 DSS能明显改善老年性痴呆模型SAM-P8小鼠的空间学习记忆能力.
目的 觀察噹歸芍藥散(DSS)對快速老化小鼠空間學習記憶能力的影響,為噹歸芍藥散治療阿爾採默病提供實驗依據.方法 選用雌性SAM-P8鼠 36隻,分成4組:預防組(3月齡)、預防對照組(3月齡)、治療組(6月齡)、治療對照組(6月齡),每組8隻.治療組以DSS(濃度為0.5g/ml)灌胃1箇月,預防組自由飲用DSS(0.02%,V/V)4箇月,對照組給予冷開水,給藥結束後行Morris水迷宮測試.結果 Morris水迷宮測試預防組、預防對照組、治療組、治療對照組的平均逃避潛伏期分彆是(41.29±20.33)s、(62.17±20.36)s、(39.67±19.60)s、(63.00±22.16)s;穿越平檯次數分彆是(6.13±2.21)次、(2.04±1.42)次、(5.41±2.13)次、(3.12±1.20)次;平檯象限遊泳距離百分比分彆是(51.14±12.38)%、(30.75±14.03)%、(47.11±9.29)%、(33.17±10.45)%.上述指標在預防組、治療組與其相應對照組比較差異有顯著性( P <0.05).結論 DSS能明顯改善老年性癡呆模型SAM-P8小鼠的空間學習記憶能力.
목적 관찰당귀작약산(DSS)대쾌속노화소서공간학습기억능력적영향,위당귀작약산치료아이채묵병제공실험의거.방법 선용자성SAM-P8서 36지,분성4조:예방조(3월령)、예방대조조(3월령)、치료조(6월령)、치료대조조(6월령),매조8지.치료조이DSS(농도위0.5g/ml)관위1개월,예방조자유음용DSS(0.02%,V/V)4개월,대조조급여랭개수,급약결속후행Morris수미궁측시.결과 Morris수미궁측시예방조、예방대조조、치료조、치료대조조적평균도피잠복기분별시(41.29±20.33)s、(62.17±20.36)s、(39.67±19.60)s、(63.00±22.16)s;천월평태차수분별시(6.13±2.21)차、(2.04±1.42)차、(5.41±2.13)차、(3.12±1.20)차;평태상한유영거리백분비분별시(51.14±12.38)%、(30.75±14.03)%、(47.11±9.29)%、(33.17±10.45)%.상술지표재예방조、치료조여기상응대조조비교차이유현저성( P <0.05).결론 DSS능명현개선노년성치태모형SAM-P8소서적공간학습기억능력.
Objective To observe the effect of Danggui Shaoyao San on the learning and memory abilities of SAMP-8 mice, as well as provide scientific basis for its treatment of Alzheimer's disease. Methods Female SAM-P8 mice were divided into treatment group(6 months,n =8), treatment control group(6 months,n =8),prevention group(3 months,n =8), prevention control group(3 months) randomly. The mice in treatment group were administrated 0.5ml DSS through stomach for 1 month.The mice in prevention group drank DSS in water(0.02%,V/V) for 3 months, then were tested learning and memory abilities by Morris water maze. Results Compared with the control group,the escape latencies[(41.29±20.33)s vs (62.17±20.36)s;(39.67±19.60)s vs (63.00±22.16)s],the times of crossing through the platform[(6.13±2.21) vs (2.04±1.42);(5.41±2.13) vs (3.12±1.20)],the percentage of swimming within the platform quadrant of pool[(51.14±12.38)% vs (30.75±14.03)%;(47.11±9.29)% vs (33.17±10.45)%] were different significantly in the treatment group and prevention group( P <0.05,P <0.01). Conclusion DSS improved the capability of learning and memory of SAM-P8 mice.
