CAJ | 학술논문

目的比较原发性结直肠癌与结直肠癌转移患者血清蛋白质谱,筛选与结直肠癌转移相关的蛋白标志物.方法采用表面增强激光解析电离-飞行时间质谱技术测定219例结直肠癌患者和健康人血清标本.将标本随机分为训练组(无转移患者57例,转移患者63例,健康人42例)和盲法测试组(无转移患者26例,转移患者31例).分析比较训练组结直肠癌转移与无转移患者及健康人血清蛋白质谱差异,筛选与转移相关的蛋白标志物,并用单盲法验证筛选出差异标志物的灵敏度与特异性.结果在平均分子质量为2000～30000范围内比较原发性结直肠癌患者与健康人血清蛋白质谱,发现31个蛋白质峰差异有统计学意义,按P值由小到大前5位的蛋白质峰分别为3240.7、9289.3、5334.4、4596.1和4792.4;比较结直肠癌转移患者与健康人血清,发现38个蛋白质峰差异有统计学意义,按P值由小到大前5位的蛋白质峰分别为3240.7、9289.3、9184.4、3191.5和9340.9;单独比较原发性结直肠癌与结直肠癌转移患者血清,筛选出两个蛋白质峰(9184.4和9340.9)差异有统计学意义,联合应用这两种蛋白可以将转移与无转移结直肠癌患者正确分组,正确分组率分别为93.6%和91.2%.经测试组盲法验证得出的灵敏度和特异性分别为90.3%和88.5%.结论用表面增强激光解析电离-飞行时间质谱技术可以监测结直肠癌转移,血清蛋白质谱中的差异蛋白可能是转移相关蛋白.
목적 비교원발성결직장암여결직장암전이환자혈청단백질보,사선여결직장암전이상관적단백표지물.방법 채용표면증강격광해석전리-비행시간질보기술측정219례결직장암환자화건강인혈청표본.장표본수궤분위훈련조(무전이환자57례,전이환자63례,건강인42례)화맹법측시조(무전이환자26례,전이환자31례).분석비교훈련조결직장암전이여무전이환자급건강인혈청단백질보차이,사선여전이상관적단백표지물,병용단맹법험증사선출차이표지물적령민도여특이성.결과 재평균분자질량위2000～30000범위내비교원발성결직장암환자여건강인혈청단백질보,발현31개단백질봉차이유통계학의의,안P치유소도대전5위적단백질봉분별위3240.7、9289.3、5334.4、4596.1화4792.4;비교결직장암전이환자여건강인혈청,발현38개단백질봉차이유통계학의의,안P치유소도대전5위적단백질봉분별위3240.7、9289.3、9184.4、3191.5화9340.9;단독비교원발성결직장암여결직장암전이환자혈청,사선출량개단백질봉(9184.4화9340.9)차이유통계학의의,연합응용저량충단백가이장전이여무전이결직장암환자정학분조,정학분조솔분별위93.6%화91.2%.경측시조맹법험증득출적령민도화특이성분별위90.3%화88.5%.결론 용표면증강격광해석전리-비행시간질보기술가이감측결직장암전이,혈청단백질보중적차이단백가능시전이상관단백.
Objective To detect biomarker proteins in relation to metastasis by comparing serum protein profiles of primary colorectal cancer patients with or without metastasis. Methods A total of 219 serum samples were analyzed using surface enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS).The samples were divided into two groups:the training group consisting of 57 primary colorectal cancer patients、63 metastatic colorectal cancer patients and 42 healthy controls,and the test group consisting of 26 primary colorectal cancer patients and 31 metastatic colorectal cancer patients.Samples in training group were analyzed to identify serum biomarker proteins which could differentiate colorectal cancer patients with or without metastasis.The sensitivity and specificity of biomarker proteins were examined by results from blind test group. Results In the m/z region of 2000～30000,31 proteins had statistically significant difference between primary colorectal cancer patients and healthy controls.The m/z of difierentiated proteins were respectively 3240.7、9289.3、5334.4、4596.1 and 4792.4 according to P value.38 proteins had statistically significant diffefence between metastatic colorectal cancer patients and healthy controls.The m/z of differentiated proteins were respectively 3240.7、9289.3、9184.4、3191.5 and 9340.9 according to P value.Only two protein peaks(9184.4 and 9340.9)were found of statistical difference between primary eolorectal cancer patients and metastatic colorectal cancer patients.The sensitivity and specificity of the combination use of the two biomarkers were respectively 90.3% and 88.5% in the test group. Conclusion SELDI-TOF-MS was helpful to find protein biomarkers with relation to metastasis in colorectal carcinoma patients.