中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
7期
836-838
,共3页
向诗琪%高进%熊秋菊%陈萍
嚮詩琪%高進%熊鞦菊%陳萍
향시기%고진%웅추국%진평
二异丙酚%海马%淀粉样β蛋白%婴儿,新生%β-分泌酶1
二異丙酚%海馬%澱粉樣β蛋白%嬰兒,新生%β-分泌酶1
이이병분%해마%정분양β단백%영인,신생%β-분비매1
Propofol%Hippocampus%Amyloid beta-protein%Infant,newborn%β-secretase 1
目的 评价异丙酚麻醉对新生大鼠海马β-分泌酶1(BACE1)表达和β淀粉样蛋白1-42(Aβ1-42)含量的影响.方法 新生7d清洁级健康SD大鼠90只,体重12 ～ 16 g,雌雄各半,采用随机数字表法,将其随机分为3组(n=30):对照组(C组)、异丙酚单次麻醉组(SP组)和异丙酚重复麻醉组(RP组).C组腹腔注射生理盐水7.5 ml/kg,1次/d,连续7 d;SP组腹腔注射生理盐水7.5 ml/kg,1次/d,连续6d,第7天腹腔注射异丙酚75 mg/kg; RP组腹腔注射异丙酚75 mg/kg,1次/d,连续7d.于第7天注射完毕后15 min,各组随机取6只大鼠,心室穿刺采血,测定血糖并行血气分析,于注射完毕后1、3和7d时各组随机取8只大鼠,分离海马,采用Western bolt法检测BACE1表达,采用ELISA法测定Aβ1-42含量.结果 与C组和SP组比较,RP组各时点大鼠海马BACE1及及Aβ1-42水平升高(P＜0.01);C组和SP组各时点大鼠海马BACE1及Aβ1-42水平比较差异无统计学意义(P＞0.05).结论 异丙酚多次麻醉新生大鼠海马BAGE1表达上调,Aβ1-42含量升高,可能是其导致远期认知功能障碍的机制之一；异丙酚单次麻醉无此作用.
目的 評價異丙酚痳醉對新生大鼠海馬β-分泌酶1(BACE1)錶達和β澱粉樣蛋白1-42(Aβ1-42)含量的影響.方法 新生7d清潔級健康SD大鼠90隻,體重12 ～ 16 g,雌雄各半,採用隨機數字錶法,將其隨機分為3組(n=30):對照組(C組)、異丙酚單次痳醉組(SP組)和異丙酚重複痳醉組(RP組).C組腹腔註射生理鹽水7.5 ml/kg,1次/d,連續7 d;SP組腹腔註射生理鹽水7.5 ml/kg,1次/d,連續6d,第7天腹腔註射異丙酚75 mg/kg; RP組腹腔註射異丙酚75 mg/kg,1次/d,連續7d.于第7天註射完畢後15 min,各組隨機取6隻大鼠,心室穿刺採血,測定血糖併行血氣分析,于註射完畢後1、3和7d時各組隨機取8隻大鼠,分離海馬,採用Western bolt法檢測BACE1錶達,採用ELISA法測定Aβ1-42含量.結果 與C組和SP組比較,RP組各時點大鼠海馬BACE1及及Aβ1-42水平升高(P＜0.01);C組和SP組各時點大鼠海馬BACE1及Aβ1-42水平比較差異無統計學意義(P＞0.05).結論 異丙酚多次痳醉新生大鼠海馬BAGE1錶達上調,Aβ1-42含量升高,可能是其導緻遠期認知功能障礙的機製之一；異丙酚單次痳醉無此作用.
목적 평개이병분마취대신생대서해마β-분비매1(BACE1)표체화β정분양단백1-42(Aβ1-42)함량적영향.방법 신생7d청길급건강SD대서90지,체중12 ～ 16 g,자웅각반,채용수궤수자표법,장기수궤분위3조(n=30):대조조(C조)、이병분단차마취조(SP조)화이병분중복마취조(RP조).C조복강주사생리염수7.5 ml/kg,1차/d,련속7 d;SP조복강주사생리염수7.5 ml/kg,1차/d,련속6d,제7천복강주사이병분75 mg/kg; RP조복강주사이병분75 mg/kg,1차/d,련속7d.우제7천주사완필후15 min,각조수궤취6지대서,심실천자채혈,측정혈당병행혈기분석,우주사완필후1、3화7d시각조수궤취8지대서,분리해마,채용Western bolt법검측BACE1표체,채용ELISA법측정Aβ1-42함량.결과 여C조화SP조비교,RP조각시점대서해마BACE1급급Aβ1-42수평승고(P＜0.01);C조화SP조각시점대서해마BACE1급Aβ1-42수평비교차이무통계학의의(P＞0.05).결론 이병분다차마취신생대서해마BAGE1표체상조,Aβ1-42함량승고,가능시기도치원기인지공능장애적궤제지일；이병분단차마취무차작용.
Objective To investigate the effect of propofol anesthesia on the expression of β-secretase 1 (BACE1) and content of anyloid beta protein 1-42 (Aβ1-42) in the neonatal rat hippocampus.Methods Ninety Sprague-Dawley rats,aged 7 days,weighing 12-16 g,were randomly divided into 3 groups ( n =30 each):control group (group C),single dose of propofol anesthesia group (group SP),and repeated doses of propofol anesthesia group (group RP).Group C received intraperitoneal normal saline 7.5 ml/kg once a day for 7 consecutive days.Group SP received normal saline 7.5 ml/kg once a day for 6 consecutive days and propofol 75 mg/kg on 7th day.Group RP received propofol 75 mg/kg once a day for 7 consecutive days.Six rats in each group were chosen at 15 min after the end of injection on 7th day and blood samples were taken from the left ventricle for determination of the blood glucose level and for blood gas analysis.Eight animals in each group were sacrificed on 1st,3rd and 7th day after the end of injection on 7th day to determine the expression of BACE1 (using Western blot) and content of Aβ1-42 in the hippocampus (by ELISA).Results Compared with groups C and SP,the expression of BACE1 was up-regulated and the content of Aβ1-42 was significantly increased at each time point in group RP ( P ＜ 0.01 ).There was no significant difference in the expression of BACE1 and content of Aβ1-42 at each time point between groups C and SP ( P ＞ 0.05).Conclusion Repeated doses of propofol up-regulate the expression of BACE1 and increase the content of Aβ1-42 in neonatal rat hippocampus,which may be one of the mechanisms by which propofol leads to long-term cognitive dysfunction.Single dose of propofol does not have the effect.
