CAJ | 학술논문

以17β-雌二醇为阳性对照组,观察不同浓度淫羊藿次甙Ⅱ对成骨细胞护骨素(OPG)表达的影响,结果发现20.ng/dl淫羊藿次甙Ⅱ上调成骨细胞OPGmRNA的表达,且优于阳性对照组(P<0.05),淫羊藿次甙Ⅱ也显著增加成骨细胞OPG分泌水平,20 ng/dl淫羊藿次甙Ⅱ组的促分泌活性显著优于阳性对照组,提示淫羊藿次甙Ⅱ作为淫羊藿甙在体内的主要代谢产物,在体外较低剂量即可显著促进成骨细胞OPG的表达,具有良好的抗骨质疏松作用.
이17β-자이순위양성대조조,관찰불동농도음양곽차대Ⅱ대성골세포호골소(OPG)표체적영향,결과발현20.ng/dl음양곽차대Ⅱ상조성골세포OPGmRNA적표체,차우우양성대조조(P<0.05),음양곽차대Ⅱ야현저증가성골세포OPG분비수평,20 ng/dl음양곽차대Ⅱ조적촉분비활성현저우우양성대조조,제시음양곽차대Ⅱ작위음양곽대재체내적주요대사산물,재체외교저제량즉가현저촉진성골세포OPG적표체,구유량호적항골질소송작용.
To explore the effect of icariside ? on osteoprotegerin(OPG) expression in osteoblasts, primary osteoblasts were cultured with different concentration of icariside ? and 17?estradiol. As a major metabolite of icarrin in vivo, icariside ? exerted a better effect on OPG expression which plays a key role in osteoporosis. 20 ng/dl icariside ? also exerted better effect on OPG secretion than that of positive control group. Consequently, icariside ?could be a candidate for osteoporosis treatment.