中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
7期
508-511
,共4页
凌向阳%祝林%韦文俊%奉建芳
凌嚮暘%祝林%韋文俊%奉建芳
릉향양%축림%위문준%봉건방
二氧化硅%粒子大小%毒性试验
二氧化硅%粒子大小%毒性試驗
이양화규%입자대소%독성시험
Silicon dioxide%Particle size%Toxicity tests
目的 观察不同粒径二氧化硅颗粒对大鼠肺组织的急性毒性.方法 将105只大鼠随机分为7组,每组15只,分别给予20、50、80、140、280、800 nm SiO2颗粒,浓度为400mg/m3.对照组不给予粉尘颗粒,各组动物在相同条件下静态吸入染尘2 h后,在24、48、72 h后处死大鼠测定肺泡灌洗液(BALF)中细胞总数、总蛋白含量和乳酸脱氢酶(LDH)活力,并进行肺组织病理观察.结果 与对照组[(0.08±0.02)g/g]比较,800 nm组除72 h总蛋白含量[(0.25±0.01)g/L]明显升高,差异有统计学意义(P<0.05)外,其他各时间各指标差异均无统计学意义(P>0.05).280mm组各时间细胞总数、总蛋白含量和LDH活力均明显高于对照组,差异有统计学意义(P<0.05).140nm组染尘后72 h细胞总数和总蛋白含量及各时点LDH活力明显高于对照组,差异有统计学意义(P<0.05).20、50、80 nm组24 h细胞总数、各时间总蛋白含量和LDH活力均明显高于对照组,差异有统计学意义(P<0.05).染尘大鼠肺组织肺泡间隔增厚,伴炎症细胞浸润.结论 在该实验条件下,较小粒径SiO2颗粒引起肺部损伤有更严重的趋势.随着染尘后时间的延长,大鼠肺部损伤均有不同程度的改善.
目的 觀察不同粒徑二氧化硅顆粒對大鼠肺組織的急性毒性.方法 將105隻大鼠隨機分為7組,每組15隻,分彆給予20、50、80、140、280、800 nm SiO2顆粒,濃度為400mg/m3.對照組不給予粉塵顆粒,各組動物在相同條件下靜態吸入染塵2 h後,在24、48、72 h後處死大鼠測定肺泡灌洗液(BALF)中細胞總數、總蛋白含量和乳痠脫氫酶(LDH)活力,併進行肺組織病理觀察.結果 與對照組[(0.08±0.02)g/g]比較,800 nm組除72 h總蛋白含量[(0.25±0.01)g/L]明顯升高,差異有統計學意義(P<0.05)外,其他各時間各指標差異均無統計學意義(P>0.05).280mm組各時間細胞總數、總蛋白含量和LDH活力均明顯高于對照組,差異有統計學意義(P<0.05).140nm組染塵後72 h細胞總數和總蛋白含量及各時點LDH活力明顯高于對照組,差異有統計學意義(P<0.05).20、50、80 nm組24 h細胞總數、各時間總蛋白含量和LDH活力均明顯高于對照組,差異有統計學意義(P<0.05).染塵大鼠肺組織肺泡間隔增厚,伴炎癥細胞浸潤.結論 在該實驗條件下,較小粒徑SiO2顆粒引起肺部損傷有更嚴重的趨勢.隨著染塵後時間的延長,大鼠肺部損傷均有不同程度的改善.
목적 관찰불동립경이양화규과립대대서폐조직적급성독성.방법 장105지대서수궤분위7조,매조15지,분별급여20、50、80、140、280、800 nm SiO2과립,농도위400mg/m3.대조조불급여분진과립,각조동물재상동조건하정태흡입염진2 h후,재24、48、72 h후처사대서측정폐포관세액(BALF)중세포총수、총단백함량화유산탈경매(LDH)활력,병진행폐조직병리관찰.결과 여대조조[(0.08±0.02)g/g]비교,800 nm조제72 h총단백함량[(0.25±0.01)g/L]명현승고,차이유통계학의의(P<0.05)외,기타각시간각지표차이균무통계학의의(P>0.05).280mm조각시간세포총수、총단백함량화LDH활력균명현고우대조조,차이유통계학의의(P<0.05).140nm조염진후72 h세포총수화총단백함량급각시점LDH활력명현고우대조조,차이유통계학의의(P<0.05).20、50、80 nm조24 h세포총수、각시간총단백함량화LDH활력균명현고우대조조,차이유통계학의의(P<0.05).염진대서폐조직폐포간격증후,반염증세포침윤.결론 재해실험조건하,교소립경SiO2과립인기폐부손상유경엄중적추세.수착염진후시간적연장,대서폐부손상균유불동정도적개선.
Objective To study the pulmonary toxicity to rats induced by different sized SiO2 particles. Methods One hundred and five male SD rats were divided into seven groups randomly according to their weight. Experimental rats were exposed to 20, 50, 80, 140, 280 and 800 nm SiO2 particles at the dose of 400 mg/m3 by inhalation for 2 hours respectively. The control group was exposed without SiO2 particles. At the 24 th, 48 th, 72 th hour after exposure, five rats were sacrificed at each time point and the total cellular scores , total protein, lactate dehydrogenase(LDH) in BALF and the histopathological changes in lungs were observed. Results The total cellular score, total protein and lactate dehydrogenase in bronchoalveolar lavage fluid (BALF) of all experimental groups were higher than the control group. Between 800 nm group and the control group, there were no significant changes in total cellular sare, total protein and LDH in BALF (P>0.05). At 48 h, the total cellular score of 280 nm group had no significantly change compared with the control group, but the total protein and LDH in BALF of 280 nm group were significantly higher than the control group (P<0.05). The total cellular score at 24 h and the total protein at 24,48 h had no significantly changes compared with the control group, but other indexes of 140 nm group were significantly higher than the control group. All the indexes of the 20, 50, 80 nm group were significantly higher than the control group (P<0.05), and higher than 800 nm group mostly time. The TCS, total protein and LDH in BALF increased firstly and then reduced. The experimental group visible part pulmonary alveolus gap varying degree proliferation accumulation, its periphery has massive phlogocyte accumulation,such as granular cells and macrophage. Conclusion Under this condition ,the SiO2 particles can cause lung damage more serious with the smaller diameter SiO2 particles. As time going on, the damage caused by SiO2 particles is not so serious as before.
